Circulating IgA from acute stage of childhood Henoch‐Schönlein purpura can enhance endothelial interleukin (IL)‐8 production through MEK/ERK signalling pathway

Summary Recently, sera from children with active Henoch‐Schönlein purpura (HSP) have been found to enhance interleukin (IL)‐8 production by human umbilical venous endothelial cells (HUVEC). To further determine the possible factor with the ability to enhance endothelial IL‐8 production in sera from acute stage of HSP, 10 children with HSP at the acute stage and 10 healthy controls were enrolled. IgA antiendothelial cell antibodies (AECA) were detected by cell‐based ELISA. Active sera with or without pretreatment with anti‐human IgA antibody, sera of controls, and immunoglobulin A (IgA) derived from sera were used to stimulate the HUVEC. The ability of these factors to enhance endothelial IL‐8 production was evaluated. Furthermore, signalling pathways were also assayed by different inhibitors, and confirmed by immunoblotting. Serum levels of IgA AECA in HPS patients at the acute stage were significantly higher than in controls (P