A novel cyclic beta-1,2-glucan mutant of Rhizobium meliloti

The periplasmic cyclic beta-1,2-glucans produced by bacteria within the Rhizobiaceae family provide function during hypo-osmotic adaptation and plant infection. In Rhizobium meliloti, these molecules are highly modified with phosphoglycerol and succinyl substituents, and it is possible that the anio...

Ausführliche Beschreibung

Gespeichert in:
Bibliographische Detailangaben
Veröffentlicht in:Journal of Bacteriology 1995-11, Vol.177 (22), p.6346-6351
Hauptverfasser: Breedveld, M.W. (University of Groningen, Haren, The Netherlands.), Hadley, J.A, Miller, K.J
Format: Artikel
Sprache:eng
Schlagworte:
Online-Zugang:Volltext
Tags: Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
Beschreibung
Zusammenfassung:The periplasmic cyclic beta-1,2-glucans produced by bacteria within the Rhizobiaceae family provide function during hypo-osmotic adaptation and plant infection. In Rhizobium meliloti, these molecules are highly modified with phosphoglycerol and succinyl substituents, and it is possible that the anionic character of these glucans is important for their functions. In the present study, we have used a thin-layer chromatographic screening method to identify a novel R. meliloti mutant specifically blocked in its ability to transfer phosphoglycerol substituents to the cyclic beta-1,2-glucan backbone. Further analysis revealed that the cyclic glucans produced by this mutant contained elevated levels of succinyl substituents. As a result, the overall anionic charge on the cyclic beta-1,2-glucans was found to be similar to that of wild-type cells. Despite this difference in cyclic beta-1,2-glucan structure, the mutant was shown to effectively nodulate alfalfa and to grow as well as wild-type cells in hypo-osmotic media
ISSN:0021-9193
1098-5530
1067-8832
DOI:10.1128/jb.177.22.6346-6351.1995