Wolfram syndrome: a clinical and molecular genetic analysis

First strand cDNA was synthesised using Superscript II (Gibco BRL) for reverse transcriptase-PCR (RT-PCR). cDNA was subcloned into pCR2.1 vector (Invitrogen) and at least three colonies sequenced for each splice variant. [...]the final WFS1 protein of the two splice variants would be expected to be...

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Veröffentlicht in:	Journal of medical genetics 2001-11, Vol.38 (11), p.e37-37
Hauptverfasser:	Eller, Philipp, Föger, Bernhard, Gander, Roland, Sauper, Teresa, Lechleitner, Monika, Finkenstedt, Gerd, Patsch, Josef R
Format:	Artikel
Sprache:	eng
Schlagworte:	Base Sequence Deoxyribonucleic acid Diabetes DNA DNA - chemistry DNA - genetics DNA Mutational Analysis Electronic Letter Female Genes Genetic Heterogeneity Humans Insulin Male Molecular weight Mutation Patients Polymorphism, Genetic Proteins RNA, Messenger - genetics RNA, Messenger - metabolism Software Wolfram Syndrome - genetics Wolfram Syndrome - pathology
Online-Zugang:	Volltext
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Beschreibung
Zusammenfassung:	First strand cDNA was synthesised using Superscript II (Gibco BRL) for reverse transcriptase-PCR (RT-PCR). cDNA was subcloned into pCR2.1 vector (Invitrogen) and at least three colonies sequenced for each splice variant. [...]the final WFS1 protein of the two splice variants would be expected to be identical. [...]no major differences in the relative density of the two splice variants was evident in the tissues examined.
ISSN:	0022-2593 1468-6244 1468-6244
DOI:	10.1136/jmg.38.11.e37