Improved resolution of PI(α1-antitrypsin) phenotypes by a large-scale immobilized pH gradient

PI variants and PI M subtypes were examined by isoelectric focusing in a preformed immobilized pH gradient. Conditions were found that permit reliable classification of PI variants and of most PI M subtypes: the pH gradient ranges from 4.3 to 4.8 on a polyacrylamide gel with a length of 20 cm and a...

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Veröffentlicht in:	American journal of human genetics 1985-09, Vol.37 (5), p.922-930
Hauptverfasser:	GORG, A, POSTEL, W, WESER, J, PATUTSCHNICK, W, CLEVE, H
Format:	Artikel
Sprache:	eng
Schlagworte:	alpha 1-Antitrypsin - analysis alpha 1-Antitrypsin - genetics Biological and medical sciences Classical genetics, quantitative genetics, hybrids Fundamental and applied biological sciences. Psychology Genetics of eukaryotes. Biological and molecular evolution Human Humans Hydrogen-Ion Concentration Isoelectric Focusing - methods Phenotype
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Zusammenfassung:	PI variants and PI M subtypes were examined by isoelectric focusing in a preformed immobilized pH gradient. Conditions were found that permit reliable classification of PI variants and of most PI M subtypes: the pH gradient ranges from 4.3 to 4.8 on a polyacrylamide gel with a length of 20 cm and a separation distance of approximately 16-17 cm (delta pH = 0.025 U/cm). The PI phenotypes observed are presented.
ISSN:	0002-9297 1537-6605