cDNA cloning and chromosomal localization (4q11-13) of a gene for statherin, a regulator of calcium in saliva

On the basis of the known amino acid sequence of statherin, a human salivary protein, mixed synthetic oligonucleotides were synthesized and used to screen a cDNA library constructed from human parotid-gland mRNA. A cDNA clone coding for statherin was isolated from this library and has been completel...

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Veröffentlicht in:	American journal of human genetics 1987-12, Vol.41 (6), p.1048-1060
Hauptverfasser:	SABATINI, L. M, CARLOCK, L. R, JOHNSON, G. W, AZEN, E. A
Format:	Artikel
Sprache:	eng
Schlagworte:	Amino Acid Sequence Animals Applied sciences Base Sequence Chromosome Mapping Chromosomes, Human, Pair 4 Cloning, Molecular Cricetinae DNA - genetics Exact sciences and technology Humans Hybrid Cells Nucleic Acid Hybridization Oligonucleotides - genetics Other techniques and industries Salivary Proteins and Peptides - genetics
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Beschreibung
Zusammenfassung:	On the basis of the known amino acid sequence of statherin, a human salivary protein, mixed synthetic oligonucleotides were synthesized and used to screen a cDNA library constructed from human parotid-gland mRNA. A cDNA clone coding for statherin was isolated from this library and has been completely sequenced. The cDNA represents a full-length (or nearly full-length) copy of an approximately 640-bp statherin mRNA. Statherin appears to be coded by a single-copy gene that maps to chromosome 4q11-4q13 when somatic-cell hybrids are used.
ISSN:	0002-9297 1537-6605