lip-Like genes in Phanerochaete sordida and Ceriporiopsis subvermispora, white rot fungi with no detectable lignin peroxidase activity

Lignin peroxidase-like genes were PCR amplified from Phanerochaete sordida and Ceriporiopsis subvermispora, fungi lacking lignin peroxidase (LiP) activity. Amplification products were highly similar to previously described LiP genes. Using reverse transcription-coupled PCR a LiP-like cDNA clone was...

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Veröffentlicht in:	Applied and Environmental Microbiology 1996-07, Vol.62 (7), p.2660-2663
Hauptverfasser:	RAJAKUMAR, S, GASKELL, J, CULLEN, D, LOBOS, S, KARAHANIAN, E, VICUNA, R
Format:	Artikel
Sprache:	eng
Schlagworte:	actividad enzimatica activite enzymatique adn Amino Acid Sequence aphyllophorales Base Sequence Basidiomycota - enzymology Basidiomycota - genetics Biological and medical sciences Biology of microorganisms of confirmed or potential industrial interest Biotechnology carie du bois Ceriporiopsis subvermispora champignon chemical composition composicion quimica composition chimique Deoxyribonucleic acid DNA DNA Primers - genetics DNA, Complementary - genetics DNA, Fungal - genetics enzymic activity Fundamental and applied biological sciences. Psychology Fungi gene genes Genes, Fungal Genetics hongos ligninas lignine lignins Microbiology microorganisme microorganismos microorganisms Mission oriented research Molecular Sequence Data nucleotide sequence pcr peroxidasas peroxidases Peroxidases - genetics peroxydase phanerochaete Polymerase Chain Reaction Polyporales - enzymology Polyporales - genetics pudricion de la madera secuencia nucleotidica Sequence Homology, Amino Acid Sequence Homology, Nucleic Acid sequence nucleotidique wood decay
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Beschreibung
Zusammenfassung:	Lignin peroxidase-like genes were PCR amplified from Phanerochaete sordida and Ceriporiopsis subvermispora, fungi lacking lignin peroxidase (LiP) activity. Amplification products were highly similar to previously described LiP genes. Using reverse transcription-coupled PCR a LiP-like cDNA clone was amplified from P. sordida RNA. In contrast, no evidence was obtained for transcription of C. subvermispora LiP genes.
ISSN:	0099-2240 1098-5336
DOI:	10.1128/AEM.62.7.2660-2663.1996