Binding of N‐(2‐bromo ethyl)‐N‐ethyl‐N1‐naphthylmethylamine HBr (SY28) to the proteins of guinea‐pig vas deferens
Summary 1 Vasa deferentia from guinea‐pigs were exposed to 14C‐SY28 HBr (5·32 × 10−6m) washed, lipid extracted, and broken up. 2 The tissue was digested with papain, hydrolyzed with 6 n HCl, and gel filtration performed. 3 Aliquots at each stage were used for paper chromatography and autoradiography...
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Veröffentlicht in: | British journal of pharmacology 1971-07, Vol.42 (3), p.428-436 |
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Format: | Artikel |
Sprache: | eng |
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Zusammenfassung: | Summary
1
Vasa deferentia from guinea‐pigs were exposed to 14C‐SY28 HBr (5·32 × 10−6m) washed, lipid extracted, and broken up.
2
The tissue was digested with papain, hydrolyzed with 6 n HCl, and gel filtration performed.
3
Aliquots at each stage were used for paper chromatography and autoradiography.
4
Three labelled spots (in addition to unbound 2‐halogenoalkylamine) were located from acid hydrolysate and a fourth from gel filtrate.
5
Selected amino‐acids were complexed with 14C‐SY28 and the procedure repeated. The RF values were compared with those of the same amino‐acids with and without 6 n HCl.
6
Histidine, aspartic acid, arginine and serine gave a significant degree of binding of 14C‐SY28.
7
The theoretical implications are discussed. |
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ISSN: | 0007-1188 1476-5381 |
DOI: | 10.1111/j.1476-5381.1971.tb07128.x |