Histidine-stimulated divalent metal uptake in human and in the erythroleukaemic cell line HEL.92.1.7

The uptake of 65 Zn by human erythrocytes was investigated in the presence of high (40 m m ) and low (5 m m ) concentrations of histidine and 0–500 μ m cobalt, nickel, manganese and zinc. Varying concentrations of metal mono- and bis-histidine complexes will be formed and the inhibition of 65 Zn uptake could be correlated with the calculated complex concentrations to investigate competition between metals. For each metal, the calculated concentrations of bis-histidine complex giving 50% inhibition of 65 Zn uptake were similar at both 5 m m and 40 m m histidine. Manganese–bis-histidine appeared to have a much higher affinity for the binding site than the other metal–bis-histidine complexes, which had similar affinities to each other. Studies of the inhibition of histidine-stimulated 54 Mn uptake by the addition of manganese confirmed that manganese–bis-histidine does act as a substrate for the transporter in a similar fashion to the other metals studied. In addition, human erythroleukaemic cells (HEL cells) were used as a model for erythroid precursor cells. l -histidine, but not d -histidine, stimulated 65 Zn uptake in a saturable fashion. The other metals competed with zinc in a similar manner to that seen in erythrocytes, and the affinity for manganese–bis-histidine was much greater than for the bis-histidine complexes of the other three metals. Both the capacity for metal transport per cell, and the affinity of the transporter for the metal–bis-histidine complexes, were much greater in the HEL cells than in the erythrocyte. It is suggested that histidine-stimulated metal transport may play a role in the supply of metals to maturing erythroid cells.