Computational identification of non‐coding RNAs in Saccharomyces cerevisiae by comparative genomics

We screened for new structural non‐coding RNAs (ncRNAs) in the genome sequence of the yeast Saccharomyces cerevisiae using computational comparative analysis of genome sequences from five related species of Saccharomyces. The screen identified 92 candidate ncRNA genes. Thirteen showed discrete trans...

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Veröffentlicht in:	Nucleic acids research 2003-07, Vol.31 (14), p.4119-4128
Hauptverfasser:	McCutcheon, John P., Eddy, Sean R.
Format:	Artikel
Sprache:	eng
Schlagworte:	Base Sequence Blotting, Northern Computational Biology - methods Conserved Sequence - genetics Databases, Nucleic Acid DNA, Complementary - chemistry DNA, Complementary - genetics DNA, Intergenic - genetics Genomics - methods Molecular Sequence Data Nucleic Acid Conformation Open Reading Frames - genetics RNA, Fungal - chemistry RNA, Fungal - genetics RNA, Fungal - metabolism RNA, Untranslated - chemistry RNA, Untranslated - genetics RNA, Untranslated - metabolism Saccharomyces - genetics Saccharomyces cerevisiae - genetics Sequence Alignment Sequence Analysis, DNA Sequence Homology, Nucleic Acid Species Specificity Transcription, Genetic
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Zusammenfassung:	We screened for new structural non‐coding RNAs (ncRNAs) in the genome sequence of the yeast Saccharomyces cerevisiae using computational comparative analysis of genome sequences from five related species of Saccharomyces. The screen identified 92 candidate ncRNA genes. Thirteen showed discrete transcripts when assayed by northern blot. Of these, eight appear to be novel ncRNAs ranging in size from 268 to 775 nt, including three new H/ACA box small nucleolar RNAs.
ISSN:	0305-1048 1362-4962 1362-4962
DOI:	10.1093/nar/gkg438