Stimulation of Dmc1-mediated DNA strand exchange by the human Rad54B protein

The process of homologous recombination is indispensable for both meiotic and mitotic cell division, and is one of the major pathways for double-strand break (DSB) repair. The human Rad54B protein, which belongs to the SWI2/SNF2 protein family, plays a role in homologous recombination, and may funct...

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Veröffentlicht in:	Nucleic acids research 2006-09, Vol.34 (16), p.4429-4437
Hauptverfasser:	Sarai, Naoyuki, Kagawa, Wataru, Kinebuchi, Takashi, Kagawa, Ako, Tanaka, Kozo, Miyagawa, Kiyoshi, Ikawa, Shukuko, Shibata, Takehiko, Kurumizaka, Hitoshi, Yokoyama, Shigeyuki
Format:	Artikel
Sprache:	eng
Schlagworte:	Adenosine Triphosphatases - chemistry Adenosine Triphosphatases - metabolism Adenosine Triphosphatases - ultrastructure Cell Cycle Proteins - chemistry Cell Cycle Proteins - metabolism Cell Cycle Proteins - ultrastructure DNA Helicases - isolation & purification DNA Helicases - metabolism DNA Helicases - ultrastructure DNA, Single-Stranded - metabolism DNA, Single-Stranded - ultrastructure DNA-Binding Proteins - chemistry DNA-Binding Proteins - metabolism DNA-Binding Proteins - ultrastructure Humans Molecular Biology Nuclear Proteins - isolation & purification Nuclear Proteins - metabolism Nuclear Proteins - ultrastructure Rad51 Recombinase - metabolism Recombinases - chemistry Recombinases - metabolism Recombinases - ultrastructure Recombination, Genetic
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Zusammenfassung:	The process of homologous recombination is indispensable for both meiotic and mitotic cell division, and is one of the major pathways for double-strand break (DSB) repair. The human Rad54B protein, which belongs to the SWI2/SNF2 protein family, plays a role in homologous recombination, and may function with the Dmc1 recombinase, a meiosis-specific Rad51 homolog. In the present study, we found that Rad54B enhanced the DNA strand-exchange activity of Dmc1 by stabilizing the Dmc1-single-stranded DNA (ssDNA) complex. Therefore, Rad54B may stimulate the Dmc1-mediated DNA strand exchange by stabilizing the nucleoprotein filament, which is formed on the ssDNA tails produced at DSB sites during homologous recombination.
ISSN:	0305-1048 1362-4962
DOI:	10.1093/nar/gkl562