Functional Architecture of the Light-Responsive Chalcone Synthase Promoter from Parsley
We have combined in vivo genomic footprinting and light-induced transient expression of chalcone synthase promoter derivatives in parsley protoplasts to identify cis sequences regulating light activation. The parsley chalcone synthase promoter contains two cis "units" that are light-respon...
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| Veröffentlicht in: | The Plant cell 1989-07, Vol.1 (7), p.707-714 |
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| container_title | The Plant cell |
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| creator | Schulze-Lefert, Paul Becker-André, Michael Schulz, Wolfgang Hahlbrock, Klaus Dangl, Jeffery L. |
| description | We have combined in vivo genomic footprinting and light-induced transient expression of chalcone synthase promoter derivatives in parsley protoplasts to identify cis sequences regulating light activation. The parsley chalcone synthase promoter contains two cis "units" that are light-responsive. Each unit is composed of short DNA stretches of approximately 50 base pairs, and each contains two in vivo footprints. One of the footprints in each unit covers a sequence that is highly conserved among other light- and stress-regulated plant genes. The other footprinted sequences in each unit are not related to each other. The TATA distal light-responsive unit is inherently weak but can compensate partially for the loss of the stronger TATA proximal unit. Levels of light-induced expression from either can be influenced by the presence of a region of approximately 100 base pairs located upstream of the TATA distal light-responsive unit. Combination of the light-responsive units and upstream region generates a synergistic response to light. We speculate that functional compensation generated by nonidentical, but sequence-related, cis units foreshadows combinatorial diversity of cognate trans factors. |
| doi_str_mv | 10.1105/tpc.1.7.707 |
| format | Article |
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The parsley chalcone synthase promoter contains two cis "units" that are light-responsive. Each unit is composed of short DNA stretches of approximately 50 base pairs, and each contains two in vivo footprints. One of the footprints in each unit covers a sequence that is highly conserved among other light- and stress-regulated plant genes. The other footprinted sequences in each unit are not related to each other. The TATA distal light-responsive unit is inherently weak but can compensate partially for the loss of the stronger TATA proximal unit. Levels of light-induced expression from either can be influenced by the presence of a region of approximately 100 base pairs located upstream of the TATA distal light-responsive unit. Combination of the light-responsive units and upstream region generates a synergistic response to light. We speculate that functional compensation generated by nonidentical, but sequence-related, cis units foreshadows combinatorial diversity of cognate trans factors.</description><identifier>ISSN: 1040-4651</identifier><identifier>EISSN: 1532-298X</identifier><identifier>DOI: 10.1105/tpc.1.7.707</identifier><identifier>PMID: 2535519</identifier><language>eng</language><publisher>United States: American Society of Plant Physiologists</publisher><subject>Acyltransferases - genetics ; Acyltransferases - metabolism ; Base Sequence ; Cells, Cultured ; Chalconoids ; Cultured cells ; DNA ; Epidermal cells ; Flavonoids ; Gene Expression Regulation ; Genes ; Light ; Molecular Sequence Data ; Parsley ; Plants - genetics ; Plasmids ; Promoter regions ; Promoter Regions, Genetic - radiation effects ; Protoplasts ; Restriction Mapping</subject><ispartof>The Plant cell, 1989-07, Vol.1 (7), p.707-714</ispartof><rights>Copyright 1989 American Society of Plant Physiologists</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c501t-2c87fb74ab9ea03329e35313b5a190dea43bc776f10a30ff2a8d858d5f007ed23</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.jstor.org/stable/pdf/3868961$$EPDF$$P50$$Gjstor$$H</linktopdf><linktohtml>$$Uhttps://www.jstor.org/stable/3868961$$EHTML$$P50$$Gjstor$$H</linktohtml><link.rule.ids>230,314,780,784,803,885,27923,27924,58016,58249</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/2535519$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Schulze-Lefert, Paul</creatorcontrib><creatorcontrib>Becker-André, Michael</creatorcontrib><creatorcontrib>Schulz, Wolfgang</creatorcontrib><creatorcontrib>Hahlbrock, Klaus</creatorcontrib><creatorcontrib>Dangl, Jeffery L.</creatorcontrib><title>Functional Architecture of the Light-Responsive Chalcone Synthase Promoter from Parsley</title><title>The Plant cell</title><addtitle>Plant Cell</addtitle><description>We have combined in vivo genomic footprinting and light-induced transient expression of chalcone synthase promoter derivatives in parsley protoplasts to identify cis sequences regulating light activation. The parsley chalcone synthase promoter contains two cis "units" that are light-responsive. Each unit is composed of short DNA stretches of approximately 50 base pairs, and each contains two in vivo footprints. One of the footprints in each unit covers a sequence that is highly conserved among other light- and stress-regulated plant genes. The other footprinted sequences in each unit are not related to each other. The TATA distal light-responsive unit is inherently weak but can compensate partially for the loss of the stronger TATA proximal unit. Levels of light-induced expression from either can be influenced by the presence of a region of approximately 100 base pairs located upstream of the TATA distal light-responsive unit. Combination of the light-responsive units and upstream region generates a synergistic response to light. We speculate that functional compensation generated by nonidentical, but sequence-related, cis units foreshadows combinatorial diversity of cognate trans factors.</description><subject>Acyltransferases - genetics</subject><subject>Acyltransferases - metabolism</subject><subject>Base Sequence</subject><subject>Cells, Cultured</subject><subject>Chalconoids</subject><subject>Cultured cells</subject><subject>DNA</subject><subject>Epidermal cells</subject><subject>Flavonoids</subject><subject>Gene Expression Regulation</subject><subject>Genes</subject><subject>Light</subject><subject>Molecular Sequence Data</subject><subject>Parsley</subject><subject>Plants - genetics</subject><subject>Plasmids</subject><subject>Promoter regions</subject><subject>Promoter Regions, Genetic - radiation effects</subject><subject>Protoplasts</subject><subject>Restriction Mapping</subject><issn>1040-4651</issn><issn>1532-298X</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1989</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNpVUU1r3DAQFaUhTdOeei7oUHop3s5Yq5V06CEsSVtYaOgH7U3I8ih28FpbSQ7sv4_bXUJymgfvY4Z5jL1BWCCC_Fh2foELtVCgnrEzlKKuaqP_PJ8xLKFariS-YC9zvgUAVGhO2WkthZRoztjvq2n0pY-jG_hF8l1fyJcpEY-Bl474pr_pSvWd8i6Oub8jvu7c4ONI_Md-LJ3LxK9T3MZCiYcZ8GuX8kD7V-wkuCHT6-M8Z7-uLn-uv1Sbb5-_ri82lZeApaq9VqFRS9cYciBEbUhIgaKRDg205Jai8UqtAoITEELtdKulbmUAUNTW4px9OuTupmZLraexJDfYXeq3Lu1tdL19yox9Z2_inUVpNKjZ__7oT_HvRLnYbZ89DYMbKU7ZKiOVlmBm4YeD0KeYc6LwsAPB_qvBzjVYtMqq_7FvH5_1oD3-febfHfjbXGJ6HFULUFbolTYrFPdzGpFo</recordid><startdate>19890701</startdate><enddate>19890701</enddate><creator>Schulze-Lefert, Paul</creator><creator>Becker-André, Michael</creator><creator>Schulz, Wolfgang</creator><creator>Hahlbrock, Klaus</creator><creator>Dangl, Jeffery L.</creator><general>American Society of Plant Physiologists</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><scope>5PM</scope></search><sort><creationdate>19890701</creationdate><title>Functional Architecture of the Light-Responsive Chalcone Synthase Promoter from Parsley</title><author>Schulze-Lefert, Paul ; Becker-André, Michael ; Schulz, Wolfgang ; Hahlbrock, Klaus ; Dangl, Jeffery L.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c501t-2c87fb74ab9ea03329e35313b5a190dea43bc776f10a30ff2a8d858d5f007ed23</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1989</creationdate><topic>Acyltransferases - genetics</topic><topic>Acyltransferases - metabolism</topic><topic>Base Sequence</topic><topic>Cells, Cultured</topic><topic>Chalconoids</topic><topic>Cultured cells</topic><topic>DNA</topic><topic>Epidermal cells</topic><topic>Flavonoids</topic><topic>Gene Expression Regulation</topic><topic>Genes</topic><topic>Light</topic><topic>Molecular Sequence Data</topic><topic>Parsley</topic><topic>Plants - genetics</topic><topic>Plasmids</topic><topic>Promoter regions</topic><topic>Promoter Regions, Genetic - radiation effects</topic><topic>Protoplasts</topic><topic>Restriction Mapping</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Schulze-Lefert, Paul</creatorcontrib><creatorcontrib>Becker-André, Michael</creatorcontrib><creatorcontrib>Schulz, Wolfgang</creatorcontrib><creatorcontrib>Hahlbrock, Klaus</creatorcontrib><creatorcontrib>Dangl, Jeffery L.</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>The Plant cell</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Schulze-Lefert, Paul</au><au>Becker-André, Michael</au><au>Schulz, Wolfgang</au><au>Hahlbrock, Klaus</au><au>Dangl, Jeffery L.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Functional Architecture of the Light-Responsive Chalcone Synthase Promoter from Parsley</atitle><jtitle>The Plant cell</jtitle><addtitle>Plant Cell</addtitle><date>1989-07-01</date><risdate>1989</risdate><volume>1</volume><issue>7</issue><spage>707</spage><epage>714</epage><pages>707-714</pages><issn>1040-4651</issn><eissn>1532-298X</eissn><abstract>We have combined in vivo genomic footprinting and light-induced transient expression of chalcone synthase promoter derivatives in parsley protoplasts to identify cis sequences regulating light activation. The parsley chalcone synthase promoter contains two cis "units" that are light-responsive. Each unit is composed of short DNA stretches of approximately 50 base pairs, and each contains two in vivo footprints. One of the footprints in each unit covers a sequence that is highly conserved among other light- and stress-regulated plant genes. The other footprinted sequences in each unit are not related to each other. The TATA distal light-responsive unit is inherently weak but can compensate partially for the loss of the stronger TATA proximal unit. Levels of light-induced expression from either can be influenced by the presence of a region of approximately 100 base pairs located upstream of the TATA distal light-responsive unit. Combination of the light-responsive units and upstream region generates a synergistic response to light. We speculate that functional compensation generated by nonidentical, but sequence-related, cis units foreshadows combinatorial diversity of cognate trans factors.</abstract><cop>United States</cop><pub>American Society of Plant Physiologists</pub><pmid>2535519</pmid><doi>10.1105/tpc.1.7.707</doi><tpages>8</tpages><oa>free_for_read</oa></addata></record> |
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| identifier | ISSN: 1040-4651 |
| ispartof | The Plant cell, 1989-07, Vol.1 (7), p.707-714 |
| issn | 1040-4651 1532-298X |
| language | eng |
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| source | MEDLINE; JSTOR Archive Collection A-Z Listing; EZB-FREE-00999 freely available EZB journals; Alma/SFX Local Collection |
| subjects | Acyltransferases - genetics Acyltransferases - metabolism Base Sequence Cells, Cultured Chalconoids Cultured cells DNA Epidermal cells Flavonoids Gene Expression Regulation Genes Light Molecular Sequence Data Parsley Plants - genetics Plasmids Promoter regions Promoter Regions, Genetic - radiation effects Protoplasts Restriction Mapping |
| title | Functional Architecture of the Light-Responsive Chalcone Synthase Promoter from Parsley |
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