Functional expression of the extraplastidial Arabidopsis thaliana oleate desaturase gene (FAD2) in Saccharomyces cerevisiae

The functional expression in yeast of the Arabidopsis thaliana FAD2 gene, encoding the extraplastidial oleate desaturase (1-acyl-2-olecyl-sn-glycero 3-phosphocholine A1 2-desaturase) is reported. Dienoic fatty acids constituted up to 11% (w/w) of the total fatty acids in transformed Saccharomyces ce...

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Veröffentlicht in:	Plant physiology (Bethesda) 1996-05, Vol.111 (1), p.223-226
Hauptverfasser:	Covello, P.S. (National Research Council, Saskatoon, Saskatchewan, Canada.), Reed, D.W
Format:	Artikel
Sprache:	eng
Schlagworte:	ACIDE GRAS ACIDOS GRASOS ACTIVIDAD ENZIMATICA ACTIVITE ENZYMATIQUE Analytical, structural and metabolic biochemistry Arabidopsis - enzymology Arabidopsis - genetics ARABIDOPSIS THALIANA Base Sequence Biochemistry Biochemistry and Enzymology Biological and medical sciences BIOSINTESIS BIOSYNTHESE Biosynthesis Cloning, Molecular DNA Primers Enzymes Enzymes and enzyme inhibitors EXPRESION GENICA EXPRESSION DES GENES Fatty Acid Desaturases - genetics Fatty Acid Desaturases - metabolism Fatty acids Fundamental and applied biological sciences. Psychology GENE Gene expression GENES Genetic vectors LECITHINE LECITINAS Lipids Molecular and cellular biology Molecular genetics Molecular Sequence Data Oxidoreductases Oxidoreductases Acting on CH-CH Group Donors OXIDORREDUCTASAS OXYDOREDUCTASE Plant cells PLANTAS TRANSGENICAS PLANTE TRANSGENIQUE SACCHAROMYCES CEREVISIAE Saccharomyces cerevisiae - genetics TRANSFERENCIA DE GENES TRANSFERT DE GENE Transformation, Genetic Unsaturated fatty acids Yeasts
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Zusammenfassung:	The functional expression in yeast of the Arabidopsis thaliana FAD2 gene, encoding the extraplastidial oleate desaturase (1-acyl-2-olecyl-sn-glycero 3-phosphocholine A1 2-desaturase) is reported. Dienoic fatty acids constituted up to 11% (w/w) of the total fatty acids in transformed Saccharomyces cerevisiae cells and were confirmed to be linoleic acid and delta 9,delta 12-hexadecadienoic acid by gas chromatography-mass spectrometry
ISSN:	0032-0889 1532-2548
DOI:	10.1104/pp.111.1.223