Characterization and modulation of EDHF‐mediated relaxations in the rat isolated superior mesenteric arterial bed

We have used the isolated, buffer‐perfused, mesenteric arterial bed of the rat (preconstricted with methoxamine or 60 mm K+) to characterize nitric oxide (NO)‐independent vasorelaxation which is thought to be mediated by the endothelium‐derived hyperpolarizing factor (EDHF). The muscarinic agonists...

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Veröffentlicht in:	British journal of pharmacology 1997-04, Vol.120 (8), p.1431-1438
Hauptverfasser:	Mcculloch, Audrey I., Bottrill, Fiona E., Randall, Michael D., Robin Hiley, C.
Format:	Artikel
Sprache:	eng
Schlagworte:	Animals Biological and medical sciences Biological Factors - pharmacology Blood vessels and receptors carbachol Carbachol - pharmacology clotrimazole Clotrimazole - pharmacology cyclic GMP Cyclic GMP - analogs & derivatives Cyclic GMP - pharmacology Drug Interactions endothelium endothelium‐derived hyperpolarizing factor (EDHF) Enzyme Inhibitors - pharmacology Fundamental and applied biological sciences. Psychology Glyburide - pharmacology In Vitro Techniques Male Mesenteric arterial bed Mesenteric Arteries - drug effects Mesenteric Arteries - physiology Muscarinic Agonists - pharmacology Muscle Relaxation - drug effects NG-Nitroarginine Methyl Ester - pharmacology NG‐nitro‐l‐arginine methyl ester (l‐NAME) nitric oxide Nitric Oxide Synthase - antagonists & inhibitors potassium channels Rats Rats, Wistar Tetraethylammonium tetraethylammonium (TEA) Tetraethylammonium Compounds - pharmacology Vertebrates: cardiovascular system
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Zusammenfassung:	We have used the isolated, buffer‐perfused, mesenteric arterial bed of the rat (preconstricted with methoxamine or 60 mm K+) to characterize nitric oxide (NO)‐independent vasorelaxation which is thought to be mediated by the endothelium‐derived hyperpolarizing factor (EDHF). The muscarinic agonists carbachol, acetylcholine (ACh) and methacholine caused dose‐related relaxations in preconstricted preparations with ED50 values of 0.18±0.04 nmol (n=8), 0.05±0.02 nmol (n=6) and 0.26±0.16 nmol (n=5), respectively. In the same preparations NG‐nitro‐l‐arginine methyl ester (l‐NAME, 100 μm) significantly (P
ISSN:	0007-1188 1476-5381
DOI:	10.1038/sj.bjp.0701066