The spot-ELISA: a sensitive in vitro method to study the immune response to hepatitis B surface antigen

We present our experience with a new sensitive in vitro method for the study of the immune response to hepatitis B surface antigen (HBsAg). This immunoenzymatic technique, called spot-ELISA, detects specific immunoglobulin production (e.g. IgG- or IgM-anti-HBs) by stimulated individual B cells in vi...

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Veröffentlicht in:	Clinical and experimental immunology 1989-10, Vol.78 (1), p.75-79
Hauptverfasser:	WISMANS, P. J, VAN HATTUM, J, DE GAST, G. C, ENDEMAN, H. J, POEL, J, STOLK, B, MAIKOE, T, MUDDE, G. C
Format:	Artikel
Sprache:	eng
Schlagworte:	Analysis of the immune response. Humoral and cellular immunity Biological and medical sciences Cells, Cultured Enzyme-Linked Immunosorbent Assay Female Fundamental and applied biological sciences. Psychology Fundamental immunology Hepatitis B Antibodies - biosynthesis Hepatitis B Surface Antigens - immunology Humans Immunobiology Immunoglobulin G - biosynthesis Immunoglobulin M - biosynthesis Leukocytes, Mononuclear - immunology Male Methods Organs and cells involved in the immune response
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Zusammenfassung:	We present our experience with a new sensitive in vitro method for the study of the immune response to hepatitis B surface antigen (HBsAg). This immunoenzymatic technique, called spot-ELISA, detects specific immunoglobulin production (e.g. IgG- or IgM-anti-HBs) by stimulated individual B cells in vitro. We have studied the immune response against HBsAg by mitogenic stimulation and subsequent spot-ELISA assay in 24 well-documented subjects with known immune status after either natural infection or vaccination with plasma-derived vaccine. Results indicate a good correlation between in vitro IgG anti-HBs spots and immune 'memory.' A possible predictive value in non-immunized and non-responder subjects awaits further confirmation.
ISSN:	0009-9104 1365-2249