Physicochemical and Light Scattering Studies on Ribosome Particles
The light scattering technique has been used to measure the molecular weight of Escherichia coli ribosomes. The 30S, 50S, and 70S components have been isolated and purified. The refractive index increment dn/dc was found to have the same value, (0.20 ±0.01) cm 3/g, for the three species. The molecul...
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| Veröffentlicht in: | Biophysical journal 1971-04, Vol.11 (4), p.370-384 |
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| creator | Scafati, Anna Reale Stornaiuolo, Maria Rosaria Novaro, Patrizia |
| description | The light scattering technique has been used to measure the molecular weight of
Escherichia coli ribosomes. The 30S, 50S, and 70S components have been isolated and purified. The refractive index increment
dn/dc was found to have the same value, (0.20
±0.01) cm
3/g, for the three species. The molecular weights are (1.0
±0.1)·10
6, (1.7
±0.1)·10
6, and (2.9
±0.3)·10
6 daltons respectively. Some information about the dimensions in solution (radius of gyration) and the interaction constant (second virial coefficient) have been obtained, and their significance is discussed. |
| doi_str_mv | 10.1016/S0006-3495(71)86221-5 |
| format | Article |
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Escherichia coli ribosomes. The 30S, 50S, and 70S components have been isolated and purified. The refractive index increment
dn/dc was found to have the same value, (0.20
±0.01) cm
3/g, for the three species. The molecular weights are (1.0
±0.1)·10
6, (1.7
±0.1)·10
6, and (2.9
±0.3)·10
6 daltons respectively. Some information about the dimensions in solution (radius of gyration) and the interaction constant (second virial coefficient) have been obtained, and their significance is discussed.</description><identifier>ISSN: 0006-3495</identifier><identifier>EISSN: 1542-0086</identifier><identifier>DOI: 10.1016/S0006-3495(71)86221-5</identifier><identifier>PMID: 4931397</identifier><language>eng</language><publisher>United States: Elsevier Inc</publisher><subject>Buffers ; Escherichia coli - cytology ; Light ; Mathematics ; Methods ; Molecular Weight ; Pancreas - enzymology ; Proteins - analysis ; Ribonucleases ; Ribosomes - analysis ; RNA, Viral - analysis ; Scattering, Radiation ; Sucrose ; Ultracentrifugation</subject><ispartof>Biophysical journal, 1971-04, Vol.11 (4), p.370-384</ispartof><rights>1971 The Biophysical Society</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c462t-428bb4b92390e046a4a0cc15dc69c97f2c1ed3abd48cf30f24b9be1253990633</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC1483971/pdf/$$EPDF$$P50$$Gpubmedcentral$$H</linktopdf><linktohtml>$$Uhttps://www.sciencedirect.com/science/article/pii/S0006349571862215$$EHTML$$P50$$Gelsevier$$Hfree_for_read</linktohtml><link.rule.ids>230,314,723,776,780,881,3537,27901,27902,53766,53768,65534</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/4931397$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Scafati, Anna Reale</creatorcontrib><creatorcontrib>Stornaiuolo, Maria Rosaria</creatorcontrib><creatorcontrib>Novaro, Patrizia</creatorcontrib><title>Physicochemical and Light Scattering Studies on Ribosome Particles</title><title>Biophysical journal</title><addtitle>Biophys J</addtitle><description>The light scattering technique has been used to measure the molecular weight of
Escherichia coli ribosomes. The 30S, 50S, and 70S components have been isolated and purified. The refractive index increment
dn/dc was found to have the same value, (0.20
±0.01) cm
3/g, for the three species. The molecular weights are (1.0
±0.1)·10
6, (1.7
±0.1)·10
6, and (2.9
±0.3)·10
6 daltons respectively. Some information about the dimensions in solution (radius of gyration) and the interaction constant (second virial coefficient) have been obtained, and their significance is discussed.</description><subject>Buffers</subject><subject>Escherichia coli - cytology</subject><subject>Light</subject><subject>Mathematics</subject><subject>Methods</subject><subject>Molecular Weight</subject><subject>Pancreas - enzymology</subject><subject>Proteins - analysis</subject><subject>Ribonucleases</subject><subject>Ribosomes - analysis</subject><subject>RNA, Viral - analysis</subject><subject>Scattering, Radiation</subject><subject>Sucrose</subject><subject>Ultracentrifugation</subject><issn>0006-3495</issn><issn>1542-0086</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1971</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkEtv1DAQxy0EKkvhI1TKCcEhMGM7D19ApeIlrUTF9m45k8muURIX21up3560u1rBidMc_o-Z-QlxgfAOAev3GwCoS6VN9abBt20tJZbVE7HCSssSoK2fitXJ8ly8SOkXAMoK8EycaaNQmWYlPl3v7pOnQDuePLmxcHNfrP12l4sNuZw5-nlbbPK-95yKMBc_fRdSmLi4djF7Gjm9FM8GNyZ-dZzn4ubL55urb-X6x9fvV5frknQtc6ll23W6M1IZYNC10w6IsOqpNmSaQRJyr1zX65YGBYNcvB0vBytjoFbqXHw41N7uu4l74jlHN9rb6CcX721w3v6rzH5nt-HOom6XV3EpeH0siOH3nlO2k0_E4-hmDvtkG23AIJrFWB2MFENKkYfTEgT7wN4-srcPYG2D9pG9rZbcxd8XnlJH2Iv-8aDzQunOc7SJPM_EvY9M2fbB_2fDH33qlNg</recordid><startdate>19710401</startdate><enddate>19710401</enddate><creator>Scafati, Anna Reale</creator><creator>Stornaiuolo, Maria Rosaria</creator><creator>Novaro, Patrizia</creator><general>Elsevier Inc</general><scope>6I.</scope><scope>AAFTH</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><scope>5PM</scope></search><sort><creationdate>19710401</creationdate><title>Physicochemical and Light Scattering Studies on Ribosome Particles</title><author>Scafati, Anna Reale ; Stornaiuolo, Maria Rosaria ; Novaro, Patrizia</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c462t-428bb4b92390e046a4a0cc15dc69c97f2c1ed3abd48cf30f24b9be1253990633</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1971</creationdate><topic>Buffers</topic><topic>Escherichia coli - cytology</topic><topic>Light</topic><topic>Mathematics</topic><topic>Methods</topic><topic>Molecular Weight</topic><topic>Pancreas - enzymology</topic><topic>Proteins - analysis</topic><topic>Ribonucleases</topic><topic>Ribosomes - analysis</topic><topic>RNA, Viral - analysis</topic><topic>Scattering, Radiation</topic><topic>Sucrose</topic><topic>Ultracentrifugation</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Scafati, Anna Reale</creatorcontrib><creatorcontrib>Stornaiuolo, Maria Rosaria</creatorcontrib><creatorcontrib>Novaro, Patrizia</creatorcontrib><collection>ScienceDirect Open Access Titles</collection><collection>Elsevier:ScienceDirect:Open Access</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Biophysical journal</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Scafati, Anna Reale</au><au>Stornaiuolo, Maria Rosaria</au><au>Novaro, Patrizia</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Physicochemical and Light Scattering Studies on Ribosome Particles</atitle><jtitle>Biophysical journal</jtitle><addtitle>Biophys J</addtitle><date>1971-04-01</date><risdate>1971</risdate><volume>11</volume><issue>4</issue><spage>370</spage><epage>384</epage><pages>370-384</pages><issn>0006-3495</issn><eissn>1542-0086</eissn><abstract>The light scattering technique has been used to measure the molecular weight of
Escherichia coli ribosomes. The 30S, 50S, and 70S components have been isolated and purified. The refractive index increment
dn/dc was found to have the same value, (0.20
±0.01) cm
3/g, for the three species. The molecular weights are (1.0
±0.1)·10
6, (1.7
±0.1)·10
6, and (2.9
±0.3)·10
6 daltons respectively. Some information about the dimensions in solution (radius of gyration) and the interaction constant (second virial coefficient) have been obtained, and their significance is discussed.</abstract><cop>United States</cop><pub>Elsevier Inc</pub><pmid>4931397</pmid><doi>10.1016/S0006-3495(71)86221-5</doi><tpages>15</tpages><oa>free_for_read</oa></addata></record> |
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| ispartof | Biophysical journal, 1971-04, Vol.11 (4), p.370-384 |
| issn | 0006-3495 1542-0086 |
| language | eng |
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| source | PubMed (Medline); MEDLINE; Cell Press Archives; Elsevier ScienceDirect Journals Complete; EZB Electronic Journals Library |
| subjects | Buffers Escherichia coli - cytology Light Mathematics Methods Molecular Weight Pancreas - enzymology Proteins - analysis Ribonucleases Ribosomes - analysis RNA, Viral - analysis Scattering, Radiation Sucrose Ultracentrifugation |
| title | Physicochemical and Light Scattering Studies on Ribosome Particles |
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