Physicochemical and Light Scattering Studies on Ribosome Particles

Physicochemical and Light Scattering Studies on Ribosome Particles

The light scattering technique has been used to measure the molecular weight of Escherichia coli ribosomes. The 30S, 50S, and 70S components have been isolated and purified. The refractive index increment dn/dc was found to have the same value, (0.20 ±0.01) cm 3/g, for the three species. The molecul...

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Veröffentlicht in:Biophysical journal 1971-04, Vol.11 (4), p.370-384
Hauptverfasser: Scafati, Anna Reale, Stornaiuolo, Maria Rosaria, Novaro, Patrizia
Format: Artikel
Sprache:eng
Schlagworte:
Buffers
Escherichia coli - cytology
Light
Mathematics
Methods
Molecular Weight
Pancreas - enzymology
Proteins - analysis
Ribonucleases
Ribosomes - analysis
RNA, Viral - analysis
Scattering, Radiation
Sucrose
Ultracentrifugation
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Zusammenfassung:The light scattering technique has been used to measure the molecular weight of Escherichia coli ribosomes. The 30S, 50S, and 70S components have been isolated and purified. The refractive index increment dn/dc was found to have the same value, (0.20 ±0.01) cm 3/g, for the three species. The molecular weights are (1.0 ±0.1)·10 6, (1.7 ±0.1)·10 6, and (2.9 ±0.3)·10 6 daltons respectively. Some information about the dimensions in solution (radius of gyration) and the interaction constant (second virial coefficient) have been obtained, and their significance is discussed.
ISSN:0006-3495
1542-0086
DOI:10.1016/S0006-3495(71)86221-5