A vector based on the SV40 origin of replication and chromosomal S/MARs replicates episomally in CHO cells

A vector based on the SV40 origin of replication and chromosomal S/MARs replicates episomally in CHO cells

We have developed an episomal replicating expression vector in which the SV40 gene coding for the large T-antigen was replaced by chromosomal scaffold/matrix attached regions. Southern analysis as well as vector rescue experiments in CHO cells and in Escherichia coli demonstrate that the vector repl...

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Veröffentlicht in:Nucleic acids research 1999-01, Vol.27 (2), p.426-428
Hauptverfasser: Piechaczek, C., Fetzer, C., Baiker, A., Bode, J., Lipps, H. J.
Format: Artikel
Sprache:eng
Schlagworte:
Animals
Antigens, Viral, Tumor - genetics
CHO Cells
Cricetinae
DNA Replication
Escherichia coli
Genetic Vectors - biosynthesis
Humans
Interferon-beta - genetics
Nuclear Proteins - genetics
Plasmids - biosynthesis
Replication Origin
Simian virus 40
Simian virus 40 - genetics
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Beschreibung
Zusammenfassung:We have developed an episomal replicating expression vector in which the SV40 gene coding for the large T-antigen was replaced by chromosomal scaffold/matrix attached regions. Southern analysis as well as vector rescue experiments in CHO cells and in Escherichia coli demonstrate that the vector replicates episomally in CHO cells. It occurs in a very low copy number in the cells and is stably maintained over more than 100 generations without selection pressure.
ISSN:0305-1048
1362-4962
DOI:10.1093/nar/27.2.426