Functional role of TRPC proteins in native systems: implications from knockout and knock-down studies
Available data on transient receptor potential channel (TRPC) protein functions indicate that these proteins represent essential constituents of agonist-activated and phospholipase C-dependent cation entry pathways in primary cells which contribute to the elevation of cytosolic Ca 2+ . In addition,...
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Veröffentlicht in: | The Journal of physiology 2005-08, Vol.567 (1), p.59-66 |
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Zusammenfassung: | Available data on transient receptor potential channel (TRPC) protein functions indicate that these proteins represent essential
constituents of agonist-activated and phospholipase C-dependent cation entry pathways in primary cells which contribute to
the elevation of cytosolic Ca 2+ . In addition, a striking number of biological functions have already been assigned to the various TRPC proteins, including
mechanosensing activity (TRPC1), chemotropic axon guidance (TRPC1 and TRPC3), pheromone sensing and the regulation of sexual
and social behaviour (TRPC2), endothelial-dependent regulation of vascular tone, endothelial permeability and neurotransmitter
release (TRPC4), axonal growth (TRPC5), modulation of smooth muscle tone in blood vessels and lung and regulation of podocyte
structure and function in the kidney (TRPC6). The lack of compounds which specifically block or activate TRPC proteins impairs
the analysis of TRPC function in primary cells. We therefore concentrate in this contribution on (i) studies of TRPC-deficient
mouse lines, (ii) data obtained by gene-silencing approaches using antisense oligonucleotides or RNA interference, (iii) expression
experiments employing dominant negative TRPC constructs, and (iv) recent data correlating mutations of TRPC genes associated
with human disease. |
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ISSN: | 0022-3751 1469-7793 |
DOI: | 10.1113/jphysiol.2005.092999 |