Functional complementation by electroporation of human BACs into mammalian fibroblast cells

Functional complementation by electroporation of human BACs into mammalian fibroblast cells

Bacterial Artificial Chromosomes (BACs) have been used to complement a metabolic defect and to transfer a drug resistance marker into mammalian cells by electroporation. The selectable markers are stable and the recipient cells have BAC DNA integrated into the chromosomes as shown by fluorescent in...

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Veröffentlicht in:Nucleic acids research 1998-02, Vol.26 (4), p.1124-1125
Hauptverfasser: Hejna, J. A., Johnstone, P. L., Kohler, S. L., Bruun, D. A., Reifsteck, C. A., Olson, S. B., Moses, R. E.
Format: Artikel
Sprache:eng
Schlagworte:
Base Sequence
Cell Line
Chromosomes, Bacterial - genetics
DNA Primers - genetics
Electroporation
Gene Transfer Techniques
Genetic Complementation Test
Genetic Markers
Genetic Techniques
Humans
In Situ Hybridization, Fluorescence
Polymerase Chain Reaction
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Beschreibung
Zusammenfassung:Bacterial Artificial Chromosomes (BACs) have been used to complement a metabolic defect and to transfer a drug resistance marker into mammalian cells by electroporation. The selectable markers are stable and the recipient cells have BAC DNA integrated into the chromosomes as shown by fluorescent in situ hybridization, PCR and Southern hybridization.
ISSN:0305-1048
1362-4962
1362-4962
DOI:10.1093/nar/26.4.1124