Colorimetric Detection of Immobilised PCR Products Generated on a Solid Support

By extending functional primers attached to a solid phase and incorporating a digoxigenin label, it is possible to visualise PCR products as discrete spots on specific regions of a solid support after colorimetric detection. The technique has been used for the detection of the point mutation associa...

Ausführliche Beschreibung

Gespeichert in:

Bibliographische Detailangaben
Veröffentlicht in:	Nucleic acids research 1997-03, Vol.25 (6), p.1313-1314
Hauptverfasser:	Lockley, Andrew K., Jones, Craigh G., Bruce, Jennifer S., Franklin, Stephen J., Bardsley, Ronald G.
Format:	Artikel
Sprache:	eng
Schlagworte:	Alleles Base Sequence Colorimetry - methods Digoxigenin DNA Primers - analysis DNA Primers - chemical synthesis DNA Primers - chemistry DNA, Antisense - chemical synthesis DNA, Antisense - chemistry Indicators and Reagents Polymerase Chain Reaction - methods
Online-Zugang:	Volltext
Tags:	Tag hinzufügen Keine Tags, Fügen Sie den ersten Tag hinzu!

Beschreibung
Zusammenfassung:	By extending functional primers attached to a solid phase and incorporating a digoxigenin label, it is possible to visualise PCR products as discrete spots on specific regions of a solid support after colorimetric detection. The technique has been used for the detection of the point mutation associated with porcine malignant hyperthermia.
ISSN:	0305-1048 1362-4962 1362-4962
DOI:	10.1093/nar/25.6.1313