Microfabricated Bioprocessor for Integrated Nanoliter-Scale Sanger DNA Sequencing

An efficient, nanoliter-scale microfabricated bioprocessor integrating all three Sanger sequencing steps, thermal cycling, sample purification, and capillary electrophoresis, has been developed and evaluated. Hybrid glass-polydimethylsiloxane (PDMS) wafer-scale construction is used to combine 250-nl...

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Veröffentlicht in:	Proceedings of the National Academy of Sciences - PNAS 2006-05, Vol.103 (19), p.7240-7245
Hauptverfasser:	Blazej, Robert G., Kumaresan, Palani, Mathies, Richard A.
Format:	Artikel
Sprache:	eng
Schlagworte:	Base Sequence Benchmarks Biological Sciences Biosensing Techniques - methods Deoxyribonucleic acid DNA DNA - genetics Electrophoresis Gels Genomes Genomics Inlets Molecular Sequence Data Nanotechnology - methods P branes Pneumatics Reagents Semiconductor wafers Sequencing
Online-Zugang:	Volltext
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