PicoGreen quantitation of DNA: effective evaluation of samples pre- or post-PCR

PicoGreen quantitation of DNA: effective evaluation of samples pre- or post-PCR

PCR has become a powerful tool for genetic analysis and many applications for gene sequence quantitation are based on this technology (1-3). Standardized reaction conditions require accurate quantitation of input DNA as well as optimization of chemical and cycling parameters. In this study we discus...

Ausführliche Beschreibung

Gespeichert in:
Bibliographische Detailangaben
Veröffentlicht in:Nucleic acids research 1996-07, Vol.24 (13), p.2623-2625
Hauptverfasser: Ahn, S J, Costa, J, Emanuel, J R
Format: Artikel
Sprache:eng
Schlagworte:
DNA - analysis
Evaluation Studies as Topic
Fluorescent Dyes
Organic Chemicals
Polymerase Chain Reaction
Sensitivity and Specificity
Spectrometry, Fluorescence
Online-Zugang:Volltext
Tags: Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
Beschreibung
Zusammenfassung:PCR has become a powerful tool for genetic analysis and many applications for gene sequence quantitation are based on this technology (1-3). Standardized reaction conditions require accurate quantitation of input DNA as well as optimization of chemical and cycling parameters. In this study we discuss PicoGreen super(TM) (Molecular Probes, Eugene, OR) fluorescence enhancement as a useful assay for template DNA quantitation and PCR product formation.
ISSN:0305-1048
1362-4962
1362-4962
DOI:10.1093/nar/24.13.2623