Eliminating gene conversion improves high-throughput genetics in Saccharomyces cerevisiae

Synthetic genetic analysis was improved by eliminating leaky expression of the HIS3 reporter and gene conversion between the HIS3 reporter and his3Delta1. Leaky expression was eliminated using 3-aminotriazole and gene conversion was eliminated by using the Schizosaccharomyces pombe his5+ gene, resul...

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Veröffentlicht in:	Genetics (Austin) 2006-01, Vol.172 (1), p.709-711
Hauptverfasser:	Daniel, Jewel A, Yoo, Jiyoun, Bettinger, Blaine T, Amberg, David C, Burke, Daniel J
Format:	Artikel
Sprache:	eng
Schlagworte:	Amitrole - metabolism Cell cycle Chromosomes, Fungal Fungal Proteins - genetics Gene Conversion Genes Genetic recombination Genome, Fungal Histidine - genetics Microarray Analysis Proteins Saccharomyces cerevisiae Saccharomyces cerevisiae - genetics Schizosaccharomyces - genetics Schizosaccharomyces pombe Yeast
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Beschreibung
Zusammenfassung:	Synthetic genetic analysis was improved by eliminating leaky expression of the HIS3 reporter and gene conversion between the HIS3 reporter and his3Delta1. Leaky expression was eliminated using 3-aminotriazole and gene conversion was eliminated by using the Schizosaccharomyces pombe his5+ gene, resulting in a 5- to 10-fold improvement in the efficiency of SGA.
ISSN:	0016-6731 1943-2631 1943-2631
DOI:	10.1534/genetics.105.047662