Human DNA polymerase β initiates DNA synthesis during long-patch repair of reduced AP sites in DNA
Simple base damages are repaired through a short‐patch base excision pathway where a single damaged nucleotide is removed and replaced. DNA polymerase β (Pol β) is responsible for the repair synthesis in this pathway and also removes a 5′‐sugar phosphate residue by catalyzing a β‐elimination reactio...
Gespeichert in:
Veröffentlicht in: | The EMBO journal 2001-03, Vol.20 (6), p.1477-1482 |
---|---|
Hauptverfasser: | , , , , |
Format: | Artikel |
Sprache: | eng |
Schlagworte: | |
Online-Zugang: | Volltext |
Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
Zusammenfassung: | Simple base damages are repaired through a short‐patch base excision pathway where a single damaged nucleotide is removed and replaced. DNA polymerase β (Pol β) is responsible for the repair synthesis in this pathway and also removes a 5′‐sugar phosphate residue by catalyzing a β‐elimination reaction. How ever, some DNA lesions that render deoxyribose resistant to β‐elimination are removed through a long‐patch repair pathway that involves strand displacement synthesis and removal of the generated flap by specific endonuclease. Three human DNA polymerases (Pol β, Pol δ and Pol ϵ) have been proposed to play a role in this pathway, however the identity of the polymerase involved and the polymerase selection mechanism are not clear. In repair reactions catalyzed by cell extracts we have used a substrate containing a reduced apurinic/apyrimidinic (AP) site resistant to β‐elimination and inhibitors that selectively affect different DNA polymerases. Using this approach we find that in human cell extracts Pol β is the major DNA polymerase incorporating the first nucleotide during repair of reduced AP sites, thus initiating long‐patch base excision repair synthesis. |
---|---|
ISSN: | 0261-4189 1460-2075 |
DOI: | 10.1093/emboj/20.6.1477 |