Quantitative analysis of associations between DNA hypermethylation, hypomethylation, and DNMT RNA levels in ovarian tumors

How hypermethylation and hypomethylation of different parts of the genome in cancer are related to each other and to DNA methyltransferase (DNMT) gene expression is ill defined. We used ovarian epithelial tumors of different malignant potential to look for associations between 5′-gene region or prom...

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Veröffentlicht in:	Oncogene 2006-04, Vol.25 (18), p.2636-2645
Hauptverfasser:	Ehrlich, M, Woods, C B, Yu, M C, Dubeau, L, Yang, F, Campan, M, Weisenberger, D J, Long, Ti, Youn, B, Fiala, E S, Laird, P W
Format:	Artikel
Sprache:	eng
Schlagworte:	Adenocarcinoma, Mucinous - genetics Adenocarcinoma, Mucinous - pathology Adolescent Adult Aged Apoptosis Biological and medical sciences Cancer Carcinoma, Endometrioid - genetics Carcinoma, Endometrioid - pathology Cell Biology Cell physiology Cell transformation and carcinogenesis. Action of oncogenes and antioncogenes Cystadenoma, Serous - genetics Cystadenoma, Serous - pathology Deoxyribonucleic acid DNA DNA (Cytosine-5-)-Methyltransferase 1 DNA (Cytosine-5-)-Methyltransferases - genetics DNA Methylation DNA methyltransferase DNA, Neoplasm DNMT1 protein E-cadherin Female Female genital diseases Fundamental and applied biological sciences. Psychology Gene expression Gene Expression Regulation, Neoplastic Genomes Gynecology. Andrology. Obstetrics Human Genetics Humans ICF syndrome Internal Medicine Isoforms Malignancy Medical sciences Medicine Medicine & Public Health Methylenetetrahydrofolate reductase Middle Aged Molecular and cellular biology Neoplasm Proteins - genetics Oncology original-article Ovarian cancer Ovarian Neoplasms - genetics Ovarian Neoplasms - pathology Ovaries Quantitative analysis Ribonucleic acid RNA RNA, Neoplasm - genetics RNA, Neoplasm - metabolism Satellite DNA Satellite RNA Tumor Suppressor Proteins Tumors
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Zusammenfassung:	How hypermethylation and hypomethylation of different parts of the genome in cancer are related to each other and to DNA methyltransferase (DNMT) gene expression is ill defined. We used ovarian epithelial tumors of different malignant potential to look for associations between 5′-gene region or promoter hypermethylation, satellite, or global DNA hypomethylation, and RNA levels for ten DNMT isoforms. In the quantitative MethyLight assay, six of the 55 examined gene loci ( LTB4R, MTHFR, CDH13, PGR, CDH1, and IGSF4) were significantly hypermethylated relative to the degree of malignancy (after adjustment for multiple comparisons; P
ISSN:	0950-9232 1476-5594
DOI:	10.1038/sj.onc.1209145