The effect of route of injection upon the development of circulating antibody in response to a variety of antigens

Ovalbumin, Escherichia coli lipopolysaccharide, and a combination of metabolic and somatic antigens of Haemonchus contortus were injected into rabbits via the intravenous, intramuscular, intradermal and intraperitoneal routes. Haemagglutination titres were obtained on untreated sera and sera after reduction and alkylation. The data suggest that the route of injection most efficient in stimulating the production of circulating antibody would depend upon whether the antigen in question elicits 19S globulin, 7S globulin or a combination of the two either in the form of sequential synthesis or a prolonged response of both types of immunoglobulins.