Capillary electrophoretic analysis of genomic DNA methylation levels

Capillary electrophoretic analysis of genomic DNA methylation levels

Changes in DNA methylation have been found in the large majority of tumors. This phenomenon includes both genome‐wide hypomethylation and gene‐ specific hypermethylation. However, the clinical relevance of either mechanism has remained contentious. In order to determine DNA methylation levels from a...

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Veröffentlicht in:Nucleic acids research 2003-01, Vol.31 (2), p.e2-e2
Hauptverfasser: Stach, Dirk, Schmitz, Oliver J., Stilgenbauer, Stephan, Benner, Axel, Döhner, Hartmut, Wiessler, Manfred, Lyko, Frank
Format: Artikel
Sprache:eng
Schlagworte:
Cytosine - metabolism
DNA Methylation
DNA, Neoplasm - genetics
DNA, Neoplasm - metabolism
Electrophoresis, Capillary - methods
Humans
Leukemia, Lymphocytic, Chronic, B-Cell - genetics
NAR Methods Online
Reproducibility of Results
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Beschreibung
Zusammenfassung:Changes in DNA methylation have been found in the large majority of tumors. This phenomenon includes both genome‐wide hypomethylation and gene‐ specific hypermethylation. However, the clinical relevance of either mechanism has remained contentious. In order to determine DNA methylation levels from a large number of clinical samples, we have established a method for accurate high‐throughput quantification of 5‐methylcytosine in genomic DNA. Our protocol requires a small amount (
ISSN:0305-1048
1362-4962
1362-4962
DOI:10.1093/nar/gng002