Presence of tumour necrosis factor or a related factor in human basophil/mast cells

The observation that mast cell products and cachectin/tumour necrosis factor (TNF) mediate similar responses suggested an investigation of cultured human basophil/mast cells for production of TNF. Using in situ hybridization and the avidin biotin-complex (ABC) immunoperoxidase method, we have demons...

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Veröffentlicht in:	Immunology 1989-03, Vol.66 (3), p.445-450
Hauptverfasser:	STEFFEN, M, ABBOUD, M, POTTER, G. K, YUNG, Y. P, MOORE, M. A. S
Format:	Artikel
Sprache:	eng
Schlagworte:	Analysis of the immune response. Humoral and cellular immunity Basophils - metabolism Biological and medical sciences Cells, Cultured Cytoplasm - metabolism Fundamental and applied biological sciences. Psychology Fundamental immunology Humans Immunobiology Mast Cells - metabolism Organs and cells involved in the immune response Tumor Necrosis Factor-alpha - metabolism
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Beschreibung
Zusammenfassung:	The observation that mast cell products and cachectin/tumour necrosis factor (TNF) mediate similar responses suggested an investigation of cultured human basophil/mast cells for production of TNF. Using in situ hybridization and the avidin biotin-complex (ABC) immunoperoxidase method, we have demonstrated the presence of TNF mRNA in the cytoplasm and TNF protein in the granules of individual human basophil/mast cells. The production of TNF by these cells could explain many of their reported functions.
ISSN:	0019-2805 1365-2567