Construction of DNA-Shuffled and Incrementally Truncated Libraries by a Mutagenic and Unidirectional Reassembly Method: Changing from a Substrate Specificity of Phospholipase to That of Lipase

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Veröffentlicht in:	Applied and Environmental Microbiology 2002-12, Vol.68 (12), p.6146-6151
Hauptverfasser:	JAE KWANG SONG, CHUNG, Bora, YOUNG HAK OH, JOON SHICK RHEE
Format:	Artikel
Sprache:	eng
Schlagworte:	Biological and medical sciences Deoxyribonucleic acid DNA DNA Shuffling - methods Enzymology and Protein Engineering Fundamental and applied biological sciences. Psychology Gene Library Lipase - genetics Lipase - metabolism Methods Mutagenesis Mutation Phospholipases A - genetics Phospholipases A - metabolism Polymerase Chain Reaction Protein Engineering - methods Proteins Serratia - enzymology Serratia - genetics Substrate Specificity
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Zusammenfassung:	Classifications Services AEM Citing Articles Google Scholar PubMed Related Content Social Bookmarking CiteULike Delicious Digg Facebook Google+ Mendeley Reddit StumbleUpon Twitter current issue Spotlights in the Current Issue AEM About AEM Subscribers Authors Reviewers Advertisers Inquiries from the Press Permissions & Commercial Reprints ASM Journals Public Access Policy AEM RSS Feeds 1752 N Street N.W. • Washington DC 20036 202.737.3600 • 202.942.9355 fax • journals@asmusa.org Print ISSN: 0099-2240 Online ISSN: 1098-5336 Copyright © 2014 by the American Society for Microbiology. For an alternate route to AEM .asm.org, visit: AEM
ISSN:	0099-2240 1098-5336
DOI:	10.1128/AEM.68.12.6146-6151.2002