Facilitated transport of prostaglandins across the blood-cerebrospinal fluid and blood-brain barriers
1. Ventriculo‐cisternal perfusions were performed on rabbits with artificial cerebrospinal fluid containing blue dextran and tritium‐labelled prostaglandin F2α ([3H]PGF2α). In order to study the nature of prostaglandin (PG) transfer across the blood—brain barrier, high concentrations of PGF2α or pot...
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Veröffentlicht in: | The Journal of physiology 1976-04, Vol.256 (2), p.273-285 |
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Sprache: | eng |
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Zusammenfassung: | 1. Ventriculo‐cisternal perfusions were performed on rabbits with artificial cerebrospinal fluid containing blue dextran and tritium‐labelled prostaglandin F2α ([3H]PGF2α). In order to study the nature of prostaglandin (PG) transfer across the blood—brain barrier, high concentrations of PGF2α or potential PG transport inhibitors were added to the perfusion fluid after the normal rate of [3H]PGF2α clearance was established.
2. The [3H]PGF2α clearance was inhibited by 10−6 to 10−3
M PGF2α, PGF2β, probenecid, iodipamide or bromcresol green but not by perchlorate.
3. The 3H content of the brain, relative to the 3H‐activity in the ventricular system, was also increased by high concentrations of PGF2α, iodipamide or bromcresol green.
4. It is concluded that the removal of PGs from the extracellular fluids of the brain is mediated by saturable, facilitated transport processes across both the choroidal and extrachoroidal regions of the blood—brain barrier system. In the case of the mammalian brain, such facilitated PG transport appears to be the primary mechanism for the termination of the action of these potent, endogenously produced autacoids. |
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ISSN: | 0022-3751 1469-7793 |
DOI: | 10.1113/jphysiol.1976.sp011325 |