Adaptation to extreme environments: macromolecular dynamics in bacteria compared in vivo by neutron scattering
Mean macromolecular dynamics was quantified in vivo by neutron scattering in psychrophile, mesophile, thermophile and hyperthermophile bacteria. Root mean square atomic fluctuation amplitudes determining macromolecular flexibility were found to be similar for each organism at its physiological tempe...
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| Veröffentlicht in: | EMBO reports 2004-01, Vol.5 (1), p.66-70 |
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| creator | Tehei, Moeava Franzetti, Bruno Madern, Dominique Ginzburg, Margaret Ginzburg, Ben Z Giudici-Orticoni, Marie-Thérèse Bruschi, Mireille Zaccai, Giuseppe |
| description | Mean macromolecular dynamics was quantified
in vivo
by neutron scattering in psychrophile, mesophile, thermophile and hyperthermophile bacteria. Root mean square atomic fluctuation amplitudes determining macromolecular flexibility were found to be similar for each organism at its physiological temperature (∼1 Å in the 0.1 ns timescale). Effective force constants determining the mean macromolecular resilience were found to increase with physiological temperature from 0.2 N/m for the psychrophiles, which grow at 4°C, to 0.6 N/m for the hyperthermophiles (85°C), indicating that the increase in stabilization free energy is dominated by enthalpic rather than entropic terms. Larger resilience allows macromolecular stability at high temperatures, while maintaining flexibility within acceptable limits for biological activity. |
| doi_str_mv | 10.1038/sj.embor.7400049 |
| format | Article |
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in vivo
by neutron scattering in psychrophile, mesophile, thermophile and hyperthermophile bacteria. Root mean square atomic fluctuation amplitudes determining macromolecular flexibility were found to be similar for each organism at its physiological temperature (∼1 Å in the 0.1 ns timescale). Effective force constants determining the mean macromolecular resilience were found to increase with physiological temperature from 0.2 N/m for the psychrophiles, which grow at 4°C, to 0.6 N/m for the hyperthermophiles (85°C), indicating that the increase in stabilization free energy is dominated by enthalpic rather than entropic terms. Larger resilience allows macromolecular stability at high temperatures, while maintaining flexibility within acceptable limits for biological activity.</description><identifier>ISSN: 1469-221X</identifier><identifier>EISSN: 1469-3178</identifier><identifier>EISSN: 1469-221X</identifier><identifier>DOI: 10.1038/sj.embor.7400049</identifier><identifier>PMID: 14710189</identifier><identifier>CODEN: ERMEAX</identifier><language>eng</language><publisher>Chichester, UK: John Wiley & Sons, Ltd</publisher><subject>Adaptation, Psychological ; Bacteria - chemistry ; Escherichia coli - chemistry ; High temperature ; Macromolecular Substances ; Models, Statistical ; Neutrons ; Oxalobacteraceae - chemistry ; Oxalobacteraceae - classification ; Physiology ; Proteus mirabilis - chemistry ; Scattering, Radiation ; Scientific Report ; Temperature ; Thermodynamics ; Thermus thermophilus - chemistry</subject><ispartof>EMBO reports, 2004-01, Vol.5 (1), p.66-70</ispartof><rights>European Molecular Biology Organization 2004</rights><rights>Copyright © 2004 European Molecular Biology Organization</rights><rights>Copyright Nature Publishing Group Jan 2004</rights><rights>Copyright © 2004, European Molecular Biology Organization 2004</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c6469-6c5aa68de33b82cdf603c7f53860cb478a9106a40a455984fffa954c44a981083</citedby><cites>FETCH-LOGICAL-c6469-6c5aa68de33b82cdf603c7f53860cb478a9106a40a455984fffa954c44a981083</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC1298960/pdf/$$EPDF$$P50$$Gpubmedcentral$$H</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC1298960/$$EHTML$$P50$$Gpubmedcentral$$H</linktohtml><link.rule.ids>230,314,723,776,780,881,1411,1427,27901,27902,45550,45551,46384,46808,53766,53768</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/14710189$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Tehei, Moeava</creatorcontrib><creatorcontrib>Franzetti, Bruno</creatorcontrib><creatorcontrib>Madern, Dominique</creatorcontrib><creatorcontrib>Ginzburg, Margaret</creatorcontrib><creatorcontrib>Ginzburg, Ben Z</creatorcontrib><creatorcontrib>Giudici-Orticoni, Marie-Thérèse</creatorcontrib><creatorcontrib>Bruschi, Mireille</creatorcontrib><creatorcontrib>Zaccai, Giuseppe</creatorcontrib><title>Adaptation to extreme environments: macromolecular dynamics in bacteria compared in vivo by neutron scattering</title><title>EMBO reports</title><addtitle>EMBO Rep</addtitle><addtitle>EMBO Rep</addtitle><description>Mean macromolecular dynamics was quantified
in vivo
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Larger resilience allows macromolecular stability at high temperatures, while maintaining flexibility within acceptable limits for biological activity.</description><subject>Adaptation, Psychological</subject><subject>Bacteria - chemistry</subject><subject>Escherichia coli - chemistry</subject><subject>High temperature</subject><subject>Macromolecular Substances</subject><subject>Models, Statistical</subject><subject>Neutrons</subject><subject>Oxalobacteraceae - chemistry</subject><subject>Oxalobacteraceae - classification</subject><subject>Physiology</subject><subject>Proteus mirabilis - chemistry</subject><subject>Scattering, Radiation</subject><subject>Scientific Report</subject><subject>Temperature</subject><subject>Thermodynamics</subject><subject>Thermus thermophilus - chemistry</subject><issn>1469-221X</issn><issn>1469-3178</issn><issn>1469-221X</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2004</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>8G5</sourceid><sourceid>BENPR</sourceid><sourceid>GUQSH</sourceid><sourceid>M2O</sourceid><recordid>eNqFkUFv1DAQhSMEomXhzglZHLjtYjuOY3NAapd2QSpFQlC4WRPHKV4SO9jJ0v33JCTqAgd6smV_8-bNvCR5SvCK4FS8jNuVaQofVjnDGDN5LzkmjMtlSnJxf75TSr4eJY9i3A5IJnPxMDkiLCeYCHmcuJMS2g466x3qPDI3XTCNQcbtbPCuMa6Lr1ADOvjG10b3NQRU7h00VkdkHSpAdyZYQNo3LQRTjo87u_Oo2CNn-m5QQVFDN1Lu-nHyoII6mifzuUg-n599Wr9dXnzYvFufXCw1Hz1znQFwUZo0LQTVZcVxqvMqSwXHumC5AEkwB4aBZZkUrKoqkBnTjIEUBIt0kbyedNu-aEyphzkC1KoNtoGwVx6s-vvH2W_q2u8UoVLIodsieTELBP-jN7FTjY3a1DU44_uoBMaCCk7vBInklEuaDeDzf8Ct74MbtqAoFhklhPMBwhM0LDzGYKpbywSrMXIVt-p35GqOfCh59ueoh4I54wGQE_DT1mZ_p6A6e3_68SBOptrYjvGZcDD9H0PLqcbGztzc9oPwXfE8zTP15XKjzvnV1SXdvFHr9Be5Ut2I</recordid><startdate>200401</startdate><enddate>200401</enddate><creator>Tehei, Moeava</creator><creator>Franzetti, Bruno</creator><creator>Madern, Dominique</creator><creator>Ginzburg, Margaret</creator><creator>Ginzburg, Ben Z</creator><creator>Giudici-Orticoni, Marie-Thérèse</creator><creator>Bruschi, Mireille</creator><creator>Zaccai, Giuseppe</creator><general>John Wiley & Sons, Ltd</general><general>Nature Publishing Group UK</general><general>Springer Nature B.V</general><scope>BSCLL</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7QL</scope><scope>7T5</scope><scope>7TM</scope><scope>7TO</scope><scope>7U9</scope><scope>7X7</scope><scope>7XB</scope><scope>88A</scope><scope>88E</scope><scope>8AO</scope><scope>8C1</scope><scope>8FD</scope><scope>8FE</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>8G5</scope><scope>ABUWG</scope><scope>AEUYN</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BHPHI</scope><scope>C1K</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FR3</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>GUQSH</scope><scope>H94</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>LK8</scope><scope>M0S</scope><scope>M1P</scope><scope>M2O</scope><scope>M7N</scope><scope>M7P</scope><scope>MBDVC</scope><scope>P64</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>Q9U</scope><scope>RC3</scope><scope>7T7</scope><scope>7X8</scope><scope>5PM</scope></search><sort><creationdate>200401</creationdate><title>Adaptation to extreme environments: macromolecular dynamics in bacteria compared in vivo by neutron scattering</title><author>Tehei, Moeava ; Franzetti, Bruno ; Madern, Dominique ; Ginzburg, Margaret ; Ginzburg, Ben Z ; Giudici-Orticoni, Marie-Thérèse ; Bruschi, Mireille ; Zaccai, Giuseppe</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c6469-6c5aa68de33b82cdf603c7f53860cb478a9106a40a455984fffa954c44a981083</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2004</creationdate><topic>Adaptation, Psychological</topic><topic>Bacteria - 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Academic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>EMBO reports</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Tehei, Moeava</au><au>Franzetti, Bruno</au><au>Madern, Dominique</au><au>Ginzburg, Margaret</au><au>Ginzburg, Ben Z</au><au>Giudici-Orticoni, Marie-Thérèse</au><au>Bruschi, Mireille</au><au>Zaccai, Giuseppe</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Adaptation to extreme environments: macromolecular dynamics in bacteria compared in vivo by neutron scattering</atitle><jtitle>EMBO reports</jtitle><stitle>EMBO Rep</stitle><addtitle>EMBO Rep</addtitle><date>2004-01</date><risdate>2004</risdate><volume>5</volume><issue>1</issue><spage>66</spage><epage>70</epage><pages>66-70</pages><issn>1469-221X</issn><eissn>1469-3178</eissn><eissn>1469-221X</eissn><coden>ERMEAX</coden><abstract>Mean macromolecular dynamics was quantified
in vivo
by neutron scattering in psychrophile, mesophile, thermophile and hyperthermophile bacteria. Root mean square atomic fluctuation amplitudes determining macromolecular flexibility were found to be similar for each organism at its physiological temperature (∼1 Å in the 0.1 ns timescale). Effective force constants determining the mean macromolecular resilience were found to increase with physiological temperature from 0.2 N/m for the psychrophiles, which grow at 4°C, to 0.6 N/m for the hyperthermophiles (85°C), indicating that the increase in stabilization free energy is dominated by enthalpic rather than entropic terms. Larger resilience allows macromolecular stability at high temperatures, while maintaining flexibility within acceptable limits for biological activity.</abstract><cop>Chichester, UK</cop><pub>John Wiley & Sons, Ltd</pub><pmid>14710189</pmid><doi>10.1038/sj.embor.7400049</doi><tpages>5</tpages><oa>free_for_read</oa></addata></record> |
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| source | Wiley Free Content; MEDLINE; Wiley Online Library Journals Frontfile Complete; Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals; PubMed Central |
| subjects | Adaptation, Psychological Bacteria - chemistry Escherichia coli - chemistry High temperature Macromolecular Substances Models, Statistical Neutrons Oxalobacteraceae - chemistry Oxalobacteraceae - classification Physiology Proteus mirabilis - chemistry Scattering, Radiation Scientific Report Temperature Thermodynamics Thermus thermophilus - chemistry |
| title | Adaptation to extreme environments: macromolecular dynamics in bacteria compared in vivo by neutron scattering |
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