Subcellular localization and adaptive up-regulation of the System A (SAT2) amino acid transporter in skeletal-muscle cells and adipocytes
The recently cloned amino acid transporter SAT2 is ubiquitously expressed and confers Na(+)-dependent transport of short-chain neutral amino acids, characteristics of the functionally defined System A transporter. Here we report the presence of SAT2 mRNA and protein in both skeletal muscle and adipo...
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| Veröffentlicht in: | Biochemical journal 2001-05, Vol.355 (Pt 3), p.563-568 |
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| container_end_page | 568 |
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| container_issue | Pt 3 |
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| container_title | Biochemical journal |
| container_volume | 355 |
| creator | Hyde, R Christie, G R Litherland, G J Hajduch, E Taylor, P M Hundal, H S |
| description | The recently cloned amino acid transporter SAT2 is ubiquitously expressed and confers Na(+)-dependent transport of short-chain neutral amino acids, characteristics of the functionally defined System A transporter. Here we report the presence of SAT2 mRNA and protein in both skeletal muscle and adipocytes, and the characterization of polyclonal antibodies directed against this transporter. SAT2 protein was present in both plasma-membrane and internal-membrane fractions derived from rat skeletal muscle and adipose tissue, L6 myotubes and 3T3-L1 adipocytes, having a localization similar to that of the glucose transporter GLUT4. Moreover, consistent with the adaptive up-regulation of System A activity following chronic amino acid deprivation, a time-dependent increase in SAT2 protein abundance was observed in amino-acid-deprived L6 myotubes and 3T3-L1 adipocytes. These studies provide the first evidence regarding the subcellular distribution and adaptive up-regulation of SAT2 protein and the characterization of molecular probes for this physiologically important transporter, the function of which is altered in several disease states. |
| doi_str_mv | 10.1042/bj3550563 |
| format | Article |
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Here we report the presence of SAT2 mRNA and protein in both skeletal muscle and adipocytes, and the characterization of polyclonal antibodies directed against this transporter. SAT2 protein was present in both plasma-membrane and internal-membrane fractions derived from rat skeletal muscle and adipose tissue, L6 myotubes and 3T3-L1 adipocytes, having a localization similar to that of the glucose transporter GLUT4. Moreover, consistent with the adaptive up-regulation of System A activity following chronic amino acid deprivation, a time-dependent increase in SAT2 protein abundance was observed in amino-acid-deprived L6 myotubes and 3T3-L1 adipocytes. 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Here we report the presence of SAT2 mRNA and protein in both skeletal muscle and adipocytes, and the characterization of polyclonal antibodies directed against this transporter. SAT2 protein was present in both plasma-membrane and internal-membrane fractions derived from rat skeletal muscle and adipose tissue, L6 myotubes and 3T3-L1 adipocytes, having a localization similar to that of the glucose transporter GLUT4. Moreover, consistent with the adaptive up-regulation of System A activity following chronic amino acid deprivation, a time-dependent increase in SAT2 protein abundance was observed in amino-acid-deprived L6 myotubes and 3T3-L1 adipocytes. These studies provide the first evidence regarding the subcellular distribution and adaptive up-regulation of SAT2 protein and the characterization of molecular probes for this physiologically important transporter, the function of which is altered in several disease states.</description><subject>Adipocytes - metabolism</subject><subject>Amino Acid Transport System A</subject><subject>Amino Acid Transport Systems</subject><subject>Animals</subject><subject>Biological Transport</subject><subject>Carrier Proteins - metabolism</subject><subject>Cells, Cultured</subject><subject>Communication</subject><subject>Male</subject><subject>Membrane Proteins - metabolism</subject><subject>Mice</subject><subject>Muscle, Skeletal - metabolism</subject><subject>Rats</subject><subject>Rats, Sprague-Dawley</subject><subject>Subcellular Fractions - metabolism</subject><subject>Up-Regulation</subject><issn>0264-6021</issn><issn>1470-8728</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2001</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNpVkcFu1DAQhi0EotvCgRdAPiF6CHjsxE4uSKsKClIlDlvOlu1MWhcnDrZTaXkD3posXRUYjTSH-fT_M_oJeQXsHbCav7d3omlYI8UTsoFasapVvH1KNozLupKMwwk5zfmOMahZzZ6TEwABa8kN-bVbrMMQlmASDdGZ4H-a4uNEzdRT05u5-Huky1wlvFmhP6s40HKLdLfPBUe6pW9322t-Ts3op0iN8z0tyUx5jqlgon6i-TsGLCZU45JdQHpwzEcHP0e3L5hfkGeDCRlfHucZ-fbp4_XF5-rq6-WXi-1V5YSCUrkO0ALv0LK1meqMk1K1DbNdq5wBbp0EJ3pQMIjesrq3roWmkQ02VphBnJEPD7rzYkfsHU7rsUHPyY8m7XU0Xv-_mfytvon3GjgHJbtV4M1RIMUfC-aiR58PH5kJ45K1Ukw2kokVPH8AXYo5JxweTYDpQ3D6MbiVff3vVX_JY1LiN4I0loY</recordid><startdate>20010501</startdate><enddate>20010501</enddate><creator>Hyde, R</creator><creator>Christie, G R</creator><creator>Litherland, G J</creator><creator>Hajduch, E</creator><creator>Taylor, P M</creator><creator>Hundal, H S</creator><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><scope>5PM</scope></search><sort><creationdate>20010501</creationdate><title>Subcellular localization and adaptive up-regulation of the System A (SAT2) amino acid transporter in skeletal-muscle cells and adipocytes</title><author>Hyde, R ; Christie, G R ; Litherland, G J ; Hajduch, E ; Taylor, P M ; Hundal, H S</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c371t-c91eb129eb0eb0079ac667850b987ca12bc61c3d171f3db04dbc815565e5b3af3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2001</creationdate><topic>Adipocytes - metabolism</topic><topic>Amino Acid Transport System A</topic><topic>Amino Acid Transport Systems</topic><topic>Animals</topic><topic>Biological Transport</topic><topic>Carrier Proteins - metabolism</topic><topic>Cells, Cultured</topic><topic>Communication</topic><topic>Male</topic><topic>Membrane Proteins - metabolism</topic><topic>Mice</topic><topic>Muscle, Skeletal - metabolism</topic><topic>Rats</topic><topic>Rats, Sprague-Dawley</topic><topic>Subcellular Fractions - metabolism</topic><topic>Up-Regulation</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Hyde, R</creatorcontrib><creatorcontrib>Christie, G R</creatorcontrib><creatorcontrib>Litherland, G J</creatorcontrib><creatorcontrib>Hajduch, E</creatorcontrib><creatorcontrib>Taylor, P M</creatorcontrib><creatorcontrib>Hundal, H S</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Biochemical journal</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Hyde, R</au><au>Christie, G R</au><au>Litherland, G J</au><au>Hajduch, E</au><au>Taylor, P M</au><au>Hundal, H S</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Subcellular localization and adaptive up-regulation of the System A (SAT2) amino acid transporter in skeletal-muscle cells and adipocytes</atitle><jtitle>Biochemical journal</jtitle><addtitle>Biochem J</addtitle><date>2001-05-01</date><risdate>2001</risdate><volume>355</volume><issue>Pt 3</issue><spage>563</spage><epage>568</epage><pages>563-568</pages><issn>0264-6021</issn><eissn>1470-8728</eissn><abstract>The recently cloned amino acid transporter SAT2 is ubiquitously expressed and confers Na(+)-dependent transport of short-chain neutral amino acids, characteristics of the functionally defined System A transporter. Here we report the presence of SAT2 mRNA and protein in both skeletal muscle and adipocytes, and the characterization of polyclonal antibodies directed against this transporter. SAT2 protein was present in both plasma-membrane and internal-membrane fractions derived from rat skeletal muscle and adipose tissue, L6 myotubes and 3T3-L1 adipocytes, having a localization similar to that of the glucose transporter GLUT4. Moreover, consistent with the adaptive up-regulation of System A activity following chronic amino acid deprivation, a time-dependent increase in SAT2 protein abundance was observed in amino-acid-deprived L6 myotubes and 3T3-L1 adipocytes. These studies provide the first evidence regarding the subcellular distribution and adaptive up-regulation of SAT2 protein and the characterization of molecular probes for this physiologically important transporter, the function of which is altered in several disease states.</abstract><cop>England</cop><pmid>11311116</pmid><doi>10.1042/bj3550563</doi><tpages>6</tpages><oa>free_for_read</oa></addata></record> |
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| subjects | Adipocytes - metabolism Amino Acid Transport System A Amino Acid Transport Systems Animals Biological Transport Carrier Proteins - metabolism Cells, Cultured Communication Male Membrane Proteins - metabolism Mice Muscle, Skeletal - metabolism Rats Rats, Sprague-Dawley Subcellular Fractions - metabolism Up-Regulation |
| title | Subcellular localization and adaptive up-regulation of the System A (SAT2) amino acid transporter in skeletal-muscle cells and adipocytes |
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