Insulin-like growth factor (IGF-I) induces myotube hypertrophy associated with an increase in anaerobic glycolysis in a clonal skeletal-muscle cell model

Insulin-like growth factor-I (IGF-I) is an important autocrine/paracrine mediator of skeletal-muscle growth and development. To develop a definitive cultured cell model of skeletal-muscle hypertrophy, C2C12 cells were stably transfected with IGF-I and clonal lines developed and evaluated. Quantitati...

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Veröffentlicht in:	Biochemical journal 1999-04, Vol.339 ( Pt 2) (2), p.443-451
Hauptverfasser:	Semsarian, C, Sutrave, P, Richmond, D R, Graham, R M
Format:	Artikel
Sprache:	eng
Schlagworte:	Animals Base Sequence Cell Differentiation - drug effects Cell Division - drug effects Cell Line DNA Primers DNA Replication - drug effects Glycolysis Hypertrophy Insulin-Like Growth Factor I - genetics Insulin-Like Growth Factor I - pharmacology Mice Models, Biological Muscle, Skeletal - drug effects Muscle, Skeletal - metabolism Muscle, Skeletal - pathology RNA, Messenger - genetics Transfection
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Zusammenfassung:	Insulin-like growth factor-I (IGF-I) is an important autocrine/paracrine mediator of skeletal-muscle growth and development. To develop a definitive cultured cell model of skeletal-muscle hypertrophy, C2C12 cells were stably transfected with IGF-I and clonal lines developed and evaluated. Quantitative morphometric analysis showed that IGF-I-transfected myotubes had a larger area (2381+/-60 micrometer2 versus 1429+/-39 micrometer2; P
ISSN:	0264-6021 1470-8728
DOI:	10.1042/0264-6021:3390443