Characterization of Saccharomyces cerevisiae deficient in expression of phospholipase D

A gene encoding phospholipase D (PLD) in Saccharomyces cerevisiae was identified. The 195 kDa product of PLD1 has 24% overall sequence identity with a plant PLD. Expression of yeast PLD activity was eliminated by one-step gene disruption. Yeast haploids lacking PLD activity were deficient in growth...

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Veröffentlicht in:	Biochemical journal 1996-02, Vol.314 (1), p.15-19
Hauptverfasser:	Ella, K.M, Dolan, J.W, Qi, C, Meier, K.E
Format:	Artikel
Sprache:	eng
Schlagworte:	Amino Acid Sequence Base Sequence cell growth Chromatography, Thin Layer Cloning, Molecular comparisons deletions diploidy DNA Primers - chemistry Gene Expression Regulation, Fungal genes Genes, Fungal haploidy Molecular Sequence Data nucleotide sequences Phenotype Phospholipase D - chemistry Phospholipase D - genetics Phospholipase D - metabolism phosphoric diester hydrolases Ploidies Saccharomyces cerevisiae Saccharomyces cerevisiae - enzymology Saccharomyces cerevisiae - genetics Saccharomyces cerevisiae - physiology sequence alignment Sequence Analysis Sequence Deletion Sequence Homology, Amino Acid Spores, Fungal - physiology sporulation strains Transformation, Genetic
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Beschreibung
Zusammenfassung:	A gene encoding phospholipase D (PLD) in Saccharomyces cerevisiae was identified. The 195 kDa product of PLD1 has 24% overall sequence identity with a plant PLD. Expression of yeast PLD activity was eliminated by one-step gene disruption. Yeast haploids lacking PLD activity were deficient in growth on non-fermentable carbon sources. Diploids lacking expression of PLD1 were unable to sporulate.
ISSN:	0264-6021 1470-8728
DOI:	10.1042/bj3140015