Somatic and germinal recombination of a direct repeat in Arabidopsis

Homologous recombination between a pair of directly repeated transgenes was studied in Arabidopsis. The test construct included two different internal, non-overlapping deletion alleles of npt (neomycin phosphotransferase) flanking an active HPT (hygromycin phosphotransferase) gene. This construct wa...

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Veröffentlicht in:Genetics (Austin) 1992-10, Vol.132 (2), p.553-566
Hauptverfasser: Assaad, F.F. (University of Munich), Signer, E.R
Format: Artikel
Sprache:eng
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Zusammenfassung:Homologous recombination between a pair of directly repeated transgenes was studied in Arabidopsis. The test construct included two different internal, non-overlapping deletion alleles of npt (neomycin phosphotransferase) flanking an active HPT (hygromycin phosphotransferase) gene. This construct was introduced into Arabidopsis by agrobacterium-mediated transformation with selection for resistance to hygromycin, and two independent single-insert lines were analyzed. Selection for active NPT by resistance to kanamycin gave both fully and partly (chimeric) recombinant seedlings. Rates for one transgenic line were estimated at 2 X 10(-5) events per division for germinal and 10(-6) events per division for somatic recombination, a much smaller difference than between meiotic and mitotic recombination in yeast. Southern analysis showed that recombinants could be formed by either crossing over or gene conversion. A surprisingly high fraction (at least 2/17) of the recombinants, however, appeared to result from the concerted action of two or more independent simple events. Some evolutionary implications are discussed
ISSN:0016-6731
1943-2631
1943-2631
DOI:10.1093/genetics/132.2.553