Afferent C fibre innervation of cat tooth pulp: confirmation by electrophysiological methods

1. The presence of afferent C fibres innervating the lower canine tooth was investigated in Nembutal-anaesthetized cats. 2. Twenty-five single fibres with conduction velocities (CVp) of less than 2.5 m/s, as calculated from the shortest response latency using monopolar electrical stimulation of the tooth, were recorded from the inferior alveolar nerve. In addition, the extradental conduction velocity (CVn) of the fibres was determined by using bipolar electrical stimulation of the trunk of the inferior alveolar nerve. 3. The mean CVp was 1.4 +/- 0.4 m/s (n = 25; range, 0.6-2.4 m/s); the mean CVn was higher, 1.7 +/- 0.9 m/s (n = 25; range, 0.6-4.0 m/s). For 20% of the fibres CVn exceeded 2.5 m/s; these were slowly conducting A delta fibres. For 80% of the fibres, however, the extradental conduction velocity was in the C fibre range. 4. The relationship between CVp (y) and CVn (x) was y = 0.66 + 0.40x, the correlation coefficient being r = 0.85. According to the present results this implies that for a reliable classification of pulpal C fibres (CVn less than or equal to 2.5 m/s) by monopolar tooth stimulation alone, CVp should be less than 1.7 m/s. 5. For twenty-three of the twenty-five fibres, one to three discrete shortenings of the response latency occurred when the intensity of the tooth stimulation was increased. When the nerve trunk itself was stimulated, a constant response latency was measured at all stimulus intensities applied. 6. For twelve fibres tested, the mean rate of electrical stimulation of the tooth, which the response followed with a constant latency, was 4.1 +/- 2.3 Hz (range, 1.5-10.0 Hz). With higher rates of stimulation the response latency increased until the fibres failed to follow each stimulus pulse. 7. Fifteen of the nineteen fibres tested responded to radiant heat stimulation of the tooth they were innervating. The mean temperature threshold was 41.4 +/- 2.7 degrees C (n = 11; range, 37.4 +/- 46.4 degrees C). 8. For eight heat-sensitive pulpal C fibres the receptive field was determined by mechanical stimulation of the exposed pulp tissue. Four C fibres developed a long-lasting on-going discharge after intense mechanical stimulation of the receptive field. 9. The discharge evoked by heat and mechanical stimulation of the tooth occluded the response evoked by simultaneously applied electrical current pulses to the nerve trunk, indicating that the same fibres were activated by both tooth and nerve stimulation.