A rapid method, using octadecasilyl-silica, for the extraction of certain peptides from tissues

Peptides can be recovered from tissues by homogenization in a carefully selected extraction medium followed by adsorption from the supernatant on a small bed of octadecasilyl-silica. Recoveries of corticotropins and somatostatin added to a variety of tissues were quantitative, and the peptides undam...

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Veröffentlicht in:	Biochemical journal 1978-12, Vol.175 (3), p.1139-1141
Hauptverfasser:	Bennett, H P, Hudson, A M, Kelly, L, McMartin, C, Purdon, G E
Format:	Artikel
Sprache:	eng
Schlagworte:	Adrenocorticotropic Hormone - isolation & purification Animals Male Methods Peptides - isolation & purification Rats Silicon Dioxide Somatostatin - isolation & purification
Online-Zugang:	Volltext
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Beschreibung
Zusammenfassung:	Peptides can be recovered from tissues by homogenization in a carefully selected extraction medium followed by adsorption from the supernatant on a small bed of octadecasilyl-silica. Recoveries of corticotropins and somatostatin added to a variety of tissues were quantitative, and the peptides undamaged as determined by high-pressure liquid chromatography.
ISSN:	0264-6021 1470-8728
DOI:	10.1042/bj1751139