Reconstitution of pig lymphocyte plasma membranes from solubilized components, with particular reference to membrane-associated immunoglobulins

Plasma membranes of lymphocytes obtained from pig mesenteric lymph nodes were reconstituted after solubilization with bile salts. The proportion by weight of immunoglobulin in the reconstituted membrane was no greater than about 5-10% of that in the original membrane. Possible reasons for the low re...

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Veröffentlicht in:	Biochemical journal 1975-11, Vol.152 (2), p.267-270
Hauptverfasser:	Chavin, S I, Holliman, A
Format:	Artikel
Sprache:	eng
Schlagworte:	Animals Detergents Electrophoresis, Polyacrylamide Gel Lymphocytes - ultrastructure Microscopy, Electron Receptors, Antigen, B-Cell Solubility Subcellular Structures Swine
Online-Zugang:	Volltext
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Beschreibung
Zusammenfassung:	Plasma membranes of lymphocytes obtained from pig mesenteric lymph nodes were reconstituted after solubilization with bile salts. The proportion by weight of immunoglobulin in the reconstituted membrane was no greater than about 5-10% of that in the original membrane. Possible reasons for the low reincorporation of immunoglobulin are discussed.
ISSN:	0264-6021 0306-3283 1470-8728
DOI:	10.1042/bj1520267