GAPDH Gene Family in Populus deltoides : Genome-Wide Identification, Structural Analysis, and Expression Analysis Under Drought Stress

Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) is an enzyme widely involved in glycolysis in animal cells and in non-metabolic processes, including apoptosis and the regulation of gene expression. GAPDH is a ubiquitous protein that plays a pivotal role in plant metabolism and handling of stress responses. However, its function in plant stress resistance remains unknown. Identification and systematic analysis of the GAPDH family in ( ) have not been performed. Bioinformatics methods were used to analyze the physicochemical characteristics, structural characteristics, phylogenetic relationships, gene structure, motif analysis, and expression of gene family members in . We identified 12 GAPDH members in . Five types of were identified: , , , , and . genes were differentially expressed in leaves, stems, and roots of 1-year-old poplar seedlings. gene transcripts showed that and were highly expressed in the leaves. In the roots, seven genes- , , , , , , and -showed significantly high expression levels. , , , and showed decreased expression under drought conditions and recovered after re-watering. These results lay the foundation for further studies on the drought stress mechanisms of