Bacterial etiologies, antimicrobial susceptibility profiles and associated factors among patients with otitis media referred to Nekemte Public Health Research and Referral Laboratory Center, Western Ethiopia: A cross-sectional study
Otitis media is among the leading causes of illnesses responsible for causing hearing problems and adding significant costs to the public health system. Bacteria are the most common causative agents for otitis media. Currently, there is little information on the prevalence and antimicrobial suscepti...
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Veröffentlicht in: | BMC microbiology 2025-01, Vol.25 (1), p.6-10, Article 6 |
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description | Otitis media is among the leading causes of illnesses responsible for causing hearing problems and adding significant costs to the public health system. Bacteria are the most common causative agents for otitis media. Currently, there is little information on the prevalence and antimicrobial susceptibility patterns of pathogenic bacterial isolates from patients with otitis media in Ethiopia.
A laboratory - based cross-sectional study was conducted from June to September 2023 among 242 patients with otitis media referred to Nekemte Public Health Research and Referral Laboratory Center. Sociodemographic and clinical data were obtained by trained nurses and/or health officers in face-to-face interviews using structured questionnaires. Middle ear discharge samples were collected by Ear, Nose and Throat (ENT) specialists (Otolaryngologist) following all aseptic techniques. Conventional culture, different biochemical tests and antimicrobial susceptibility testing were performed for all the isolated bacteria. Reference strains were used as a positive and negative controls. The data were checked for completeness and consistency, entered into EpiData version 4.6.06 and analyzed by SPSS version 25. Logistic regression analysis was performed to determine the associated factors of otitis media. Adjusted odds ratio was used to determine strength of association. Statistical significance was obtained at p-value of below 0.05. The data were interpreted using graphs, tables, and results statements.
A total of 242 middle ear discharge samples were collected and cultured from which 212 (87.6%) were culture positive. A total of 228 pathogenic bacterial isolates were recovered. The predominant bacterial isolates were S. aureus 92 (40.4%) followed by P. aeruginosa 33 (14.5%) and E. coli 24 (10.5%). One hundred and fifty-one (66.2%) bacterial pathogens were multidrug resistant. Piperacillin-tazobactam and tobramycin were relatively common drugs to which most of the isolates were susceptible while they were most resistant to ampicillin and tetracycline. Purulent discharge (p-value = 0.001), middle ear discharge ≥ 14 days (P-value = 0.000) and a history of active/passive smoking (P-value = 0.043) were significantly associated with otitis media.
The prevalence of bacterial pathogens, most of which were multidrug-resistant, was high among patients with otitis media. A significant association was observed with purulent ear discharge, chronic otitis media, and passive or active smoking |
doi_str_mv | 10.1186/s12866-024-03664-z |
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A laboratory - based cross-sectional study was conducted from June to September 2023 among 242 patients with otitis media referred to Nekemte Public Health Research and Referral Laboratory Center. Sociodemographic and clinical data were obtained by trained nurses and/or health officers in face-to-face interviews using structured questionnaires. Middle ear discharge samples were collected by Ear, Nose and Throat (ENT) specialists (Otolaryngologist) following all aseptic techniques. Conventional culture, different biochemical tests and antimicrobial susceptibility testing were performed for all the isolated bacteria. Reference strains were used as a positive and negative controls. The data were checked for completeness and consistency, entered into EpiData version 4.6.06 and analyzed by SPSS version 25. Logistic regression analysis was performed to determine the associated factors of otitis media. Adjusted odds ratio was used to determine strength of association. Statistical significance was obtained at p-value of below 0.05. The data were interpreted using graphs, tables, and results statements.
A total of 242 middle ear discharge samples were collected and cultured from which 212 (87.6%) were culture positive. A total of 228 pathogenic bacterial isolates were recovered. The predominant bacterial isolates were S. aureus 92 (40.4%) followed by P. aeruginosa 33 (14.5%) and E. coli 24 (10.5%). One hundred and fifty-one (66.2%) bacterial pathogens were multidrug resistant. Piperacillin-tazobactam and tobramycin were relatively common drugs to which most of the isolates were susceptible while they were most resistant to ampicillin and tetracycline. Purulent discharge (p-value = 0.001), middle ear discharge ≥ 14 days (P-value = 0.000) and a history of active/passive smoking (P-value = 0.043) were significantly associated with otitis media.
The prevalence of bacterial pathogens, most of which were multidrug-resistant, was high among patients with otitis media. A significant association was observed with purulent ear discharge, chronic otitis media, and passive or active smoking. Choosing the proper antibiotic for the treatment of bacterial infection is crucial.</description><identifier>ISSN: 1471-2180</identifier><identifier>EISSN: 1471-2180</identifier><identifier>DOI: 10.1186/s12866-024-03664-z</identifier><identifier>PMID: 39773118</identifier><language>eng</language><publisher>England: BioMed Central Ltd</publisher><subject>Adolescent ; Adult ; Ampicillin ; Anti-Bacterial Agents - pharmacology ; Anti-infective agents ; Antibiotics ; Antimicrobial agents ; Antimicrobial resistance ; Bacteria ; Bacteria - classification ; Bacteria - drug effects ; Bacteria - isolation & purification ; Bacteria, Pathogenic ; Bacterial diseases ; Biochemical tests ; Causes of ; Child ; Child, Preschool ; Chronic infection ; Cross-Sectional Studies ; Culture ; Data collection ; Dosage and administration ; Drug therapy ; E coli ; Ear diseases ; Eardrum ; Ears & hearing ; Ethiopia - epidemiology ; Female ; Health facilities ; Hearing loss ; Humans ; Infant ; Inflammation ; Laboratories ; Male ; Medical research ; Medicine, Experimental ; Microbial Sensitivity Tests ; Microbiology ; Middle Aged ; Middle ear ; Multidrug resistance ; Otitis media ; Otitis Media - epidemiology ; Otitis Media - microbiology ; Passive smoking ; Pathogens ; Patients ; Physiological aspects ; Piperacillin-tazobactam ; Prevalence ; Public health ; Quality standards ; Questionnaires ; Referral and Consultation - statistics & numerical data ; Regression analysis ; Software ; Statistical analysis ; Streptococcus infections ; Supervisors ; Tobramycin ; Young Adult</subject><ispartof>BMC microbiology, 2025-01, Vol.25 (1), p.6-10, Article 6</ispartof><rights>2024. The Author(s).</rights><rights>COPYRIGHT 2025 BioMed Central Ltd.</rights><rights>2025. This work is licensed under http://creativecommons.org/licenses/by-nc-nd/4.0/ (the “License”). Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.</rights><rights>The Author(s) 2024 2024</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><cites>FETCH-LOGICAL-c435t-397ae3d04ab57cb74bf376fa41349f0dbe7065e425401f4462697c04a6b12d393</cites><orcidid>0009-0000-6082-0703</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC11705670/pdf/$$EPDF$$P50$$Gpubmedcentral$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC11705670/$$EHTML$$P50$$Gpubmedcentral$$Hfree_for_read</linktohtml><link.rule.ids>230,314,723,776,780,860,881,2096,27901,27902,53766,53768</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/39773118$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Guteta, Endalu Tesfaye</creatorcontrib><creatorcontrib>Abdi, Fedasan Alemu</creatorcontrib><creatorcontrib>Feyisa, Seifu Gizaw</creatorcontrib><creatorcontrib>Kinfu, Betrearon Sileshi</creatorcontrib><creatorcontrib>Tafesse, Tadese Bekele</creatorcontrib><title>Bacterial etiologies, antimicrobial susceptibility profiles and associated factors among patients with otitis media referred to Nekemte Public Health Research and Referral Laboratory Center, Western Ethiopia: A cross-sectional study</title><title>BMC microbiology</title><addtitle>BMC Microbiol</addtitle><description>Otitis media is among the leading causes of illnesses responsible for causing hearing problems and adding significant costs to the public health system. Bacteria are the most common causative agents for otitis media. Currently, there is little information on the prevalence and antimicrobial susceptibility patterns of pathogenic bacterial isolates from patients with otitis media in Ethiopia.
A laboratory - based cross-sectional study was conducted from June to September 2023 among 242 patients with otitis media referred to Nekemte Public Health Research and Referral Laboratory Center. Sociodemographic and clinical data were obtained by trained nurses and/or health officers in face-to-face interviews using structured questionnaires. Middle ear discharge samples were collected by Ear, Nose and Throat (ENT) specialists (Otolaryngologist) following all aseptic techniques. Conventional culture, different biochemical tests and antimicrobial susceptibility testing were performed for all the isolated bacteria. Reference strains were used as a positive and negative controls. The data were checked for completeness and consistency, entered into EpiData version 4.6.06 and analyzed by SPSS version 25. Logistic regression analysis was performed to determine the associated factors of otitis media. Adjusted odds ratio was used to determine strength of association. Statistical significance was obtained at p-value of below 0.05. The data were interpreted using graphs, tables, and results statements.
A total of 242 middle ear discharge samples were collected and cultured from which 212 (87.6%) were culture positive. A total of 228 pathogenic bacterial isolates were recovered. The predominant bacterial isolates were S. aureus 92 (40.4%) followed by P. aeruginosa 33 (14.5%) and E. coli 24 (10.5%). One hundred and fifty-one (66.2%) bacterial pathogens were multidrug resistant. Piperacillin-tazobactam and tobramycin were relatively common drugs to which most of the isolates were susceptible while they were most resistant to ampicillin and tetracycline. Purulent discharge (p-value = 0.001), middle ear discharge ≥ 14 days (P-value = 0.000) and a history of active/passive smoking (P-value = 0.043) were significantly associated with otitis media.
The prevalence of bacterial pathogens, most of which were multidrug-resistant, was high among patients with otitis media. A significant association was observed with purulent ear discharge, chronic otitis media, and passive or active smoking. Choosing the proper antibiotic for the treatment of bacterial infection is crucial.</description><subject>Adolescent</subject><subject>Adult</subject><subject>Ampicillin</subject><subject>Anti-Bacterial Agents - pharmacology</subject><subject>Anti-infective agents</subject><subject>Antibiotics</subject><subject>Antimicrobial agents</subject><subject>Antimicrobial resistance</subject><subject>Bacteria</subject><subject>Bacteria - classification</subject><subject>Bacteria - drug effects</subject><subject>Bacteria - isolation & purification</subject><subject>Bacteria, Pathogenic</subject><subject>Bacterial diseases</subject><subject>Biochemical tests</subject><subject>Causes of</subject><subject>Child</subject><subject>Child, Preschool</subject><subject>Chronic infection</subject><subject>Cross-Sectional Studies</subject><subject>Culture</subject><subject>Data collection</subject><subject>Dosage and administration</subject><subject>Drug therapy</subject><subject>E coli</subject><subject>Ear diseases</subject><subject>Eardrum</subject><subject>Ears & hearing</subject><subject>Ethiopia - epidemiology</subject><subject>Female</subject><subject>Health facilities</subject><subject>Hearing loss</subject><subject>Humans</subject><subject>Infant</subject><subject>Inflammation</subject><subject>Laboratories</subject><subject>Male</subject><subject>Medical research</subject><subject>Medicine, Experimental</subject><subject>Microbial Sensitivity Tests</subject><subject>Microbiology</subject><subject>Middle Aged</subject><subject>Middle ear</subject><subject>Multidrug resistance</subject><subject>Otitis media</subject><subject>Otitis Media - epidemiology</subject><subject>Otitis Media - microbiology</subject><subject>Passive smoking</subject><subject>Pathogens</subject><subject>Patients</subject><subject>Physiological aspects</subject><subject>Piperacillin-tazobactam</subject><subject>Prevalence</subject><subject>Public health</subject><subject>Quality standards</subject><subject>Questionnaires</subject><subject>Referral and Consultation - statistics & numerical data</subject><subject>Regression analysis</subject><subject>Software</subject><subject>Statistical analysis</subject><subject>Streptococcus infections</subject><subject>Supervisors</subject><subject>Tobramycin</subject><subject>Young Adult</subject><issn>1471-2180</issn><issn>1471-2180</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2025</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>BENPR</sourceid><sourceid>DOA</sourceid><recordid>eNptk1Fv0zAQxyMEYmPwBXhAlngBaRl27MQpL6hMg02qABUQj9YlubQeaVxsB-g-MR-DSzvGilAeHNm_-5_vf74keSz4iRBl8SKIrCyKlGcq5bIoVHp1JzkUSos0EyW_e-v_IHkQwiXnQpdS308O5ERrSRqHya_XUEf0FjqG0brOLSyGYwZ9tCtbe1eNJ2EINa6jrWxn44atvWtth4GohkEIrrYQsWEtSTlP2yvXL9gaosU-BvbDxiVz0UYb2AobC8xji95TRHTsHX7FVUT2Yag6W7NzhI7wOQYEXy-3KeZbnO4xg8p5oBwbdkrS6I_ZFwy09uwsLq1bW3jJpoyuHUIasKaC-vH6cWg2D5N7LXQBH12vR8nnN2efTs_T2fu3F6fTWVormceUnAGUDVdQ5bqutKpaqYsWlJBq0vKmQs2LHFWWKy5apYqsmOia8KISWSMn8ii52Ok2Di7N2tsV-I1xYM12w_mFAR9t3aFptIY2LwWAzhVwLHXblpOKmlpq1VCrjpJXO631UJFzNdVMNuyJ7p_0dmkW7rsRQvO80JwUnl0rePdtIK_MylIvuw56dEMwUuSy1FIVOaFP_0Ev3eDJvy01lplp-ZdaAFVg-9ZR4noUNdMyy8pSlPmY9uQ_FH0N0qtyPY7vZz_g-V4AMRF_xgUMIZiLj_N9Ntux2z7TW7oxRHAzDobZDYahwTDbwTBXFPTktpU3IX8mQf4GACINLA</recordid><startdate>20250107</startdate><enddate>20250107</enddate><creator>Guteta, Endalu Tesfaye</creator><creator>Abdi, Fedasan Alemu</creator><creator>Feyisa, Seifu Gizaw</creator><creator>Kinfu, Betrearon Sileshi</creator><creator>Tafesse, Tadese Bekele</creator><general>BioMed Central Ltd</general><general>BioMed Central</general><general>BMC</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>ISR</scope><scope>3V.</scope><scope>7QL</scope><scope>7T7</scope><scope>7U9</scope><scope>7X7</scope><scope>7XB</scope><scope>88E</scope><scope>8FD</scope><scope>8FE</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABUWG</scope><scope>AEUYN</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BHPHI</scope><scope>C1K</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FR3</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>H94</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>LK8</scope><scope>M0S</scope><scope>M1P</scope><scope>M7N</scope><scope>M7P</scope><scope>P64</scope><scope>PIMPY</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope><scope>7X8</scope><scope>5PM</scope><scope>DOA</scope><orcidid>https://orcid.org/0009-0000-6082-0703</orcidid></search><sort><creationdate>20250107</creationdate><title>Bacterial etiologies, antimicrobial susceptibility profiles and associated factors among patients with otitis media referred to Nekemte Public Health Research and Referral Laboratory Center, Western Ethiopia: A cross-sectional study</title><author>Guteta, Endalu Tesfaye ; Abdi, Fedasan Alemu ; Feyisa, Seifu Gizaw ; Kinfu, Betrearon Sileshi ; Tafesse, Tadese Bekele</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c435t-397ae3d04ab57cb74bf376fa41349f0dbe7065e425401f4462697c04a6b12d393</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2025</creationdate><topic>Adolescent</topic><topic>Adult</topic><topic>Ampicillin</topic><topic>Anti-Bacterial Agents - 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Academic</collection><collection>PubMed Central (Full Participant titles)</collection><collection>DOAJ Directory of Open Access Journals</collection><jtitle>BMC microbiology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Guteta, Endalu Tesfaye</au><au>Abdi, Fedasan Alemu</au><au>Feyisa, Seifu Gizaw</au><au>Kinfu, Betrearon Sileshi</au><au>Tafesse, Tadese Bekele</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Bacterial etiologies, antimicrobial susceptibility profiles and associated factors among patients with otitis media referred to Nekemte Public Health Research and Referral Laboratory Center, Western Ethiopia: A cross-sectional study</atitle><jtitle>BMC microbiology</jtitle><addtitle>BMC Microbiol</addtitle><date>2025-01-07</date><risdate>2025</risdate><volume>25</volume><issue>1</issue><spage>6</spage><epage>10</epage><pages>6-10</pages><artnum>6</artnum><issn>1471-2180</issn><eissn>1471-2180</eissn><abstract>Otitis media is among the leading causes of illnesses responsible for causing hearing problems and adding significant costs to the public health system. Bacteria are the most common causative agents for otitis media. Currently, there is little information on the prevalence and antimicrobial susceptibility patterns of pathogenic bacterial isolates from patients with otitis media in Ethiopia.
A laboratory - based cross-sectional study was conducted from June to September 2023 among 242 patients with otitis media referred to Nekemte Public Health Research and Referral Laboratory Center. Sociodemographic and clinical data were obtained by trained nurses and/or health officers in face-to-face interviews using structured questionnaires. Middle ear discharge samples were collected by Ear, Nose and Throat (ENT) specialists (Otolaryngologist) following all aseptic techniques. Conventional culture, different biochemical tests and antimicrobial susceptibility testing were performed for all the isolated bacteria. Reference strains were used as a positive and negative controls. The data were checked for completeness and consistency, entered into EpiData version 4.6.06 and analyzed by SPSS version 25. Logistic regression analysis was performed to determine the associated factors of otitis media. Adjusted odds ratio was used to determine strength of association. Statistical significance was obtained at p-value of below 0.05. The data were interpreted using graphs, tables, and results statements.
A total of 242 middle ear discharge samples were collected and cultured from which 212 (87.6%) were culture positive. A total of 228 pathogenic bacterial isolates were recovered. The predominant bacterial isolates were S. aureus 92 (40.4%) followed by P. aeruginosa 33 (14.5%) and E. coli 24 (10.5%). One hundred and fifty-one (66.2%) bacterial pathogens were multidrug resistant. Piperacillin-tazobactam and tobramycin were relatively common drugs to which most of the isolates were susceptible while they were most resistant to ampicillin and tetracycline. Purulent discharge (p-value = 0.001), middle ear discharge ≥ 14 days (P-value = 0.000) and a history of active/passive smoking (P-value = 0.043) were significantly associated with otitis media.
The prevalence of bacterial pathogens, most of which were multidrug-resistant, was high among patients with otitis media. A significant association was observed with purulent ear discharge, chronic otitis media, and passive or active smoking. Choosing the proper antibiotic for the treatment of bacterial infection is crucial.</abstract><cop>England</cop><pub>BioMed Central Ltd</pub><pmid>39773118</pmid><doi>10.1186/s12866-024-03664-z</doi><tpages>10</tpages><orcidid>https://orcid.org/0009-0000-6082-0703</orcidid><oa>free_for_read</oa></addata></record> |
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language | eng |
recordid | cdi_pubmedcentral_primary_oai_pubmedcentral_nih_gov_11705670 |
source | MEDLINE; Springer Nature - Complete Springer Journals; DOAJ Directory of Open Access Journals; Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals; PubMed Central; PubMed Central Open Access; Springer Nature OA Free Journals |
subjects | Adolescent Adult Ampicillin Anti-Bacterial Agents - pharmacology Anti-infective agents Antibiotics Antimicrobial agents Antimicrobial resistance Bacteria Bacteria - classification Bacteria - drug effects Bacteria - isolation & purification Bacteria, Pathogenic Bacterial diseases Biochemical tests Causes of Child Child, Preschool Chronic infection Cross-Sectional Studies Culture Data collection Dosage and administration Drug therapy E coli Ear diseases Eardrum Ears & hearing Ethiopia - epidemiology Female Health facilities Hearing loss Humans Infant Inflammation Laboratories Male Medical research Medicine, Experimental Microbial Sensitivity Tests Microbiology Middle Aged Middle ear Multidrug resistance Otitis media Otitis Media - epidemiology Otitis Media - microbiology Passive smoking Pathogens Patients Physiological aspects Piperacillin-tazobactam Prevalence Public health Quality standards Questionnaires Referral and Consultation - statistics & numerical data Regression analysis Software Statistical analysis Streptococcus infections Supervisors Tobramycin Young Adult |
title | Bacterial etiologies, antimicrobial susceptibility profiles and associated factors among patients with otitis media referred to Nekemte Public Health Research and Referral Laboratory Center, Western Ethiopia: A cross-sectional study |
url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-02-13T19%3A33%3A01IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-gale_doaj_&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Bacterial%20etiologies,%20antimicrobial%20susceptibility%20profiles%20and%20associated%20factors%20among%20patients%20with%20otitis%20media%20referred%20to%20Nekemte%20Public%20Health%20Research%20and%20Referral%20Laboratory%20Center,%20Western%20Ethiopia:%20A%20cross-sectional%20study&rft.jtitle=BMC%20microbiology&rft.au=Guteta,%20Endalu%20Tesfaye&rft.date=2025-01-07&rft.volume=25&rft.issue=1&rft.spage=6&rft.epage=10&rft.pages=6-10&rft.artnum=6&rft.issn=1471-2180&rft.eissn=1471-2180&rft_id=info:doi/10.1186/s12866-024-03664-z&rft_dat=%3Cgale_doaj_%3EA822881850%3C/gale_doaj_%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=3152697273&rft_id=info:pmid/39773118&rft_galeid=A822881850&rft_doaj_id=oai_doaj_org_article_d77af581aa754a0e87ff89b286874d78&rfr_iscdi=true |