14‐3‐3ε conditional knockout mice exhibit defects in the development of the epidermis

The epidermis is a stratified epithelium that functions as the first line of defense against pathogenic invasion and acts as a barrier preventing water loss. In this study, we aimed to decipher the role of 14‐3‐3ε in the development of the epidermis. We report that loss of 14‐3‐3ε in the epidermis of juvenile and adult mice reduces cell division in the basal layer and increases the percentage of cells with multiple centrosomes, leading to a reduction in the thickness of the basal and stratified layers. We also demonstrate a decrease in the expression of differentiation markers, although no gross morphological defects in the skin or adverse effects on the survival of the mice were observed. These results suggest that loss of 14‐3‐3ε in the epidermis may lead to defects in proliferation and differentiation. A knockout of 14‐3‐3ε in the basal layer of the epidermis in mice leads to a decrease in proliferation and the thickness of the epidermis and decreased expression of differentiation markers. The knockout cells also show centrosome amplification, suggesting that centrosome amplification leads to decreased proliferation and subsequent thinning of the skin in these mice.