Isolation and characterization of a sigB deletion mutant of Staphylococcus aureus
The sigB gene of Staphylococcus aureus, coding for the alternate sigma factor B, has been deleted by allelic replacement mutagenesis. The mutant grew as well as the parent in vitro, although it was deficient in clumping factor, coagulase, and pigment. In two murine and one rat infection model the mu...
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| Veröffentlicht in: | Infection and immunity 1999-07, Vol.67 (7), p.3667-3669 |
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| container_end_page | 3669 |
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| container_issue | 7 |
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| container_title | Infection and immunity |
| container_volume | 67 |
| creator | NICHOLAS, R. O TONG LI MCDEVITT, D MARRA, A SUCOLOSKI, T. S DEMARSH, P. L GENTRY, D. R |
| description | The sigB gene of Staphylococcus aureus, coding for the alternate sigma factor B, has been deleted by allelic replacement mutagenesis. The mutant grew as well as the parent in vitro, although it was deficient in clumping factor, coagulase, and pigment. In two murine and one rat infection model the mutant showed no reduction in virulence. |
| doi_str_mv | 10.1128/IAI.67.7.3667-3669.1999 |
| format | Article |
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In two murine and one rat infection model the mutant showed no reduction in virulence.</description><identifier>ISSN: 0019-9567</identifier><identifier>EISSN: 1098-5522</identifier><identifier>DOI: 10.1128/IAI.67.7.3667-3669.1999</identifier><identifier>PMID: 10377157</identifier><identifier>CODEN: INFIBR</identifier><language>eng</language><publisher>Washington, DC: American Society for Microbiology</publisher><subject>Animals ; Bacterial Proteins - genetics ; Bacteriology ; Biological and medical sciences ; clumping factor ; coagulase ; Fundamental and applied biological sciences. 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I.</contributor><creatorcontrib>NICHOLAS, R. O</creatorcontrib><creatorcontrib>TONG LI</creatorcontrib><creatorcontrib>MCDEVITT, D</creatorcontrib><creatorcontrib>MARRA, A</creatorcontrib><creatorcontrib>SUCOLOSKI, T. S</creatorcontrib><creatorcontrib>DEMARSH, P. L</creatorcontrib><creatorcontrib>GENTRY, D. R</creatorcontrib><title>Isolation and characterization of a sigB deletion mutant of Staphylococcus aureus</title><title>Infection and immunity</title><addtitle>Infect Immun</addtitle><description>The sigB gene of Staphylococcus aureus, coding for the alternate sigma factor B, has been deleted by allelic replacement mutagenesis. The mutant grew as well as the parent in vitro, although it was deficient in clumping factor, coagulase, and pigment. In two murine and one rat infection model the mutant showed no reduction in virulence.</description><subject>Animals</subject><subject>Bacterial Proteins - genetics</subject><subject>Bacteriology</subject><subject>Biological and medical sciences</subject><subject>clumping factor</subject><subject>coagulase</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Gene Deletion</subject><subject>Mice</subject><subject>Microbiology</subject><subject>Molecular and Cellular Pathogenesis</subject><subject>Pathogenicity, virulence, toxins, bacteriocins, pyrogens, host-bacteria relations, miscellaneous strains</subject><subject>Rats</subject><subject>sigB gene</subject><subject>Sigma Factor - genetics</subject><subject>Staphylococcal Infections - microbiology</subject><subject>Staphylococcus aureus</subject><subject>Staphylococcus aureus - genetics</subject><subject>Staphylococcus aureus - pathogenicity</subject><subject>Virulence - genetics</subject><issn>0019-9567</issn><issn>1098-5522</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1999</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNpVkU1v1DAQhi1ERZfCX4AcELcEf8Se-MChVHysVKlCwNmaTJxuUDZe7ASp_fU47Aray4xm5nnt0byMvRa8EkI277aX28pABZUyBsocbCWstU_YRnDblFpL-ZRtOBe2tNrAOXue0s9c1nXdPGPngisAoWHDvm5TGHEewlTg1BW0w4g0-zjcH5uhL7BIw-2HovOj_9vaLzNO8zr5NuNhdzcGCkRLKnCJfkkv2FmPY_IvT_mC_fj08fvVl_L65vP26vK6pBrkXJJoMG-MRFKi0V41IAW00vgGrJRKodWt5l4S-pp63tm-7XroqNWq5bxTF-z98d3D0u59R36aI47uEIc9xjsXcHCPJ9Owc7fhtxPCaCOz_u1JH8OvxafZ7YdEfhxx8mFJToACka-UQTiCFENK0ff__hDcrW647IYz4MCtbqzButWNrHz1cMUHuuP5M_DmBGAiHPuIEw3pP9cYqyVXfwB08pXB</recordid><startdate>19990701</startdate><enddate>19990701</enddate><creator>NICHOLAS, R. 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R</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c472t-c18a999acc22a65e387217b26e8792233a95b50e2cae4cf0d9fbdf7dcb53b00d3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1999</creationdate><topic>Animals</topic><topic>Bacterial Proteins - genetics</topic><topic>Bacteriology</topic><topic>Biological and medical sciences</topic><topic>clumping factor</topic><topic>coagulase</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Gene Deletion</topic><topic>Mice</topic><topic>Microbiology</topic><topic>Molecular and Cellular Pathogenesis</topic><topic>Pathogenicity, virulence, toxins, bacteriocins, pyrogens, host-bacteria relations, miscellaneous strains</topic><topic>Rats</topic><topic>sigB gene</topic><topic>Sigma Factor - genetics</topic><topic>Staphylococcal Infections - microbiology</topic><topic>Staphylococcus aureus</topic><topic>Staphylococcus aureus - genetics</topic><topic>Staphylococcus aureus - pathogenicity</topic><topic>Virulence - genetics</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>NICHOLAS, R. O</creatorcontrib><creatorcontrib>TONG LI</creatorcontrib><creatorcontrib>MCDEVITT, D</creatorcontrib><creatorcontrib>MARRA, A</creatorcontrib><creatorcontrib>SUCOLOSKI, T. S</creatorcontrib><creatorcontrib>DEMARSH, P. L</creatorcontrib><creatorcontrib>GENTRY, D. R</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Environmental Sciences and Pollution Management</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Infection and immunity</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>NICHOLAS, R. O</au><au>TONG LI</au><au>MCDEVITT, D</au><au>MARRA, A</au><au>SUCOLOSKI, T. S</au><au>DEMARSH, P. L</au><au>GENTRY, D. R</au><au>Tuomanen, E. I.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Isolation and characterization of a sigB deletion mutant of Staphylococcus aureus</atitle><jtitle>Infection and immunity</jtitle><addtitle>Infect Immun</addtitle><date>1999-07-01</date><risdate>1999</risdate><volume>67</volume><issue>7</issue><spage>3667</spage><epage>3669</epage><pages>3667-3669</pages><issn>0019-9567</issn><eissn>1098-5522</eissn><coden>INFIBR</coden><abstract>The sigB gene of Staphylococcus aureus, coding for the alternate sigma factor B, has been deleted by allelic replacement mutagenesis. The mutant grew as well as the parent in vitro, although it was deficient in clumping factor, coagulase, and pigment. 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| ispartof | Infection and immunity, 1999-07, Vol.67 (7), p.3667-3669 |
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| source | American Society for Microbiology; MEDLINE; Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals; PubMed Central |
| subjects | Animals Bacterial Proteins - genetics Bacteriology Biological and medical sciences clumping factor coagulase Fundamental and applied biological sciences. Psychology Gene Deletion Mice Microbiology Molecular and Cellular Pathogenesis Pathogenicity, virulence, toxins, bacteriocins, pyrogens, host-bacteria relations, miscellaneous strains Rats sigB gene Sigma Factor - genetics Staphylococcal Infections - microbiology Staphylococcus aureus Staphylococcus aureus - genetics Staphylococcus aureus - pathogenicity Virulence - genetics |
| title | Isolation and characterization of a sigB deletion mutant of Staphylococcus aureus |
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