Differential oxygen sensitivity of calcium channels in rabbit smooth muscle cells of conduit and resistance pulmonary arteries

1. Calcium currents were recorded from smooth muscle cells dispersed from conduit and resistance rabbit pulmonary arteries. We tested the hypothesis that Ca2+ channel activity was regulated by environmental O2 tension. 2. Conduit (proximal) and resistance (distal) myocytes differ in their Ca2+ chann...

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Veröffentlicht in:The Journal of physiology 1996-03, Vol.491 (Pt 2), p.511-518
Hauptverfasser: A Franco-Obregón, J López-Barneo
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J López-Barneo
description 1. Calcium currents were recorded from smooth muscle cells dispersed from conduit and resistance rabbit pulmonary arteries. We tested the hypothesis that Ca2+ channel activity was regulated by environmental O2 tension. 2. Conduit (proximal) and resistance (distal) myocytes differ in their Ca2+ channel density and responses to low PO2. Ca2+ current density in distal myocytes (20.7 +/- 7.4 pA pF-1, n = 10) is almost twice the value in proximal myocytes (12.6 +/- 5.5 pA pF-1, n = 39). In proximal myocytes, the predominant response to reductions in PO2 is inhibition of the calcium current (n = 12) at membrane potentials below 0 mV, whereas potentiation of current amplitude is observed in distal myocytes (n = 24). 3. Hypoxia also produces opposite shifts in the conductance-voltage relationships along the voltage axis. The average displacements induced by low PO2 are +5.05 +/- 2.98 mV (n = 5) in proximal myocytes and -6.06 +/- 2.45 (n = 10) in distal myocytes. 4. These findings demonstrate longitudinal differences in Ca2+ channel density and O2 sensitivity in myocytes along the pulmonary arterial tree. These results may help to understand the differential reactivity to hypoxia of the pulmonary vasculature: vasodilatation in conduit arteries and vasoconstriction in resistance vessels.
doi_str_mv 10.1113/jphysiol.1996.sp021235
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Calcium currents were recorded from smooth muscle cells dispersed from conduit and resistance rabbit pulmonary arteries. We tested the hypothesis that Ca2+ channel activity was regulated by environmental O2 tension. 2. Conduit (proximal) and resistance (distal) myocytes differ in their Ca2+ channel density and responses to low PO2. Ca2+ current density in distal myocytes (20.7 +/- 7.4 pA pF-1, n = 10) is almost twice the value in proximal myocytes (12.6 +/- 5.5 pA pF-1, n = 39). In proximal myocytes, the predominant response to reductions in PO2 is inhibition of the calcium current (n = 12) at membrane potentials below 0 mV, whereas potentiation of current amplitude is observed in distal myocytes (n = 24). 3. Hypoxia also produces opposite shifts in the conductance-voltage relationships along the voltage axis. The average displacements induced by low PO2 are +5.05 +/- 2.98 mV (n = 5) in proximal myocytes and -6.06 +/- 2.45 (n = 10) in distal myocytes. 4. These findings demonstrate longitudinal differences in Ca2+ channel density and O2 sensitivity in myocytes along the pulmonary arterial tree. These results may help to understand the differential reactivity to hypoxia of the pulmonary vasculature: vasodilatation in conduit arteries and vasoconstriction in resistance vessels.</description><identifier>ISSN: 0022-3751</identifier><identifier>EISSN: 1469-7793</identifier><identifier>DOI: 10.1113/jphysiol.1996.sp021235</identifier><identifier>PMID: 8866874</identifier><language>eng</language><publisher>England: The Physiological Society</publisher><subject>Animals ; Calcium Channels - drug effects ; Calcium Channels - physiology ; Hypoxia - physiopathology ; Male ; Membrane Potentials - drug effects ; Membrane Potentials - physiology ; Muscle, Smooth, Vascular - drug effects ; Muscle, Smooth, Vascular - physiology ; Oxygen - pharmacology ; Pulmonary Artery - drug effects ; Rabbits</subject><ispartof>The Journal of physiology, 1996-03, Vol.491 (Pt 2), p.511-518</ispartof><rights>1996 The Physiological Society</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c6021-dce61e2c348d73f2f4ae3ff309af7bfffd4fbbf7ed890bda463c67878d6b81523</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC1158745/pdf/$$EPDF$$P50$$Gpubmedcentral$$H</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC1158745/$$EHTML$$P50$$Gpubmedcentral$$H</linktohtml><link.rule.ids>230,314,727,780,784,885,1417,27924,27925,45574,45575,53791,53793</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/8866874$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>A Franco-Obregón</creatorcontrib><creatorcontrib>J López-Barneo</creatorcontrib><title>Differential oxygen sensitivity of calcium channels in rabbit smooth muscle cells of conduit and resistance pulmonary arteries</title><title>The Journal of physiology</title><addtitle>J Physiol</addtitle><description>1. Calcium currents were recorded from smooth muscle cells dispersed from conduit and resistance rabbit pulmonary arteries. We tested the hypothesis that Ca2+ channel activity was regulated by environmental O2 tension. 2. Conduit (proximal) and resistance (distal) myocytes differ in their Ca2+ channel density and responses to low PO2. Ca2+ current density in distal myocytes (20.7 +/- 7.4 pA pF-1, n = 10) is almost twice the value in proximal myocytes (12.6 +/- 5.5 pA pF-1, n = 39). In proximal myocytes, the predominant response to reductions in PO2 is inhibition of the calcium current (n = 12) at membrane potentials below 0 mV, whereas potentiation of current amplitude is observed in distal myocytes (n = 24). 3. Hypoxia also produces opposite shifts in the conductance-voltage relationships along the voltage axis. The average displacements induced by low PO2 are +5.05 +/- 2.98 mV (n = 5) in proximal myocytes and -6.06 +/- 2.45 (n = 10) in distal myocytes. 4. These findings demonstrate longitudinal differences in Ca2+ channel density and O2 sensitivity in myocytes along the pulmonary arterial tree. These results may help to understand the differential reactivity to hypoxia of the pulmonary vasculature: vasodilatation in conduit arteries and vasoconstriction in resistance vessels.</description><subject>Animals</subject><subject>Calcium Channels - drug effects</subject><subject>Calcium Channels - physiology</subject><subject>Hypoxia - physiopathology</subject><subject>Male</subject><subject>Membrane Potentials - drug effects</subject><subject>Membrane Potentials - physiology</subject><subject>Muscle, Smooth, Vascular - drug effects</subject><subject>Muscle, Smooth, Vascular - physiology</subject><subject>Oxygen - pharmacology</subject><subject>Pulmonary Artery - drug effects</subject><subject>Rabbits</subject><issn>0022-3751</issn><issn>1469-7793</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1996</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqNkU1v1DAYhC0EKtuFnwDyCaFKWezEiZMLUinfqkQP5Ww5zuuNq8RebKclF347jrKt4II4WfI8M_J4EHpJyY5SWry5OfRzMG7Y0aapduFAcpoX5SO0oaxqMs6b4jHaEJLnWcFL-hSdhnBDCC1I05ygk7quqpqzDfr13mgNHmw0csDu57wHiwPYYKK5NXHGTmMlB2WmEateWgtDwMZiL9vWRBxG52KPxymoAbCCIamLw9luSrK0HfYQTIjSKsCHaRidlX7G0kfwBsIz9ETLIcDz47lF3z9-uL74nF1--_Tl4vwyU1UqlnUKKgq5Kljd8ULnmkkotE5lpOat1rpjum01h65uSNtJVhWq4jWvu6qtaZkXW_R2zT1M7QgpzkYvB3HwZkzPEU4a8bdiTS_27lZQWqZ_KlPAq2OAdz8mCFGMJix9pQU3BcFrxtIsC_j6nyDlpKG8ZDVLaLWiyrsQPOiH91AilpHF_chiGVncj5yML_5s82A7rpr0d6t-ZwaY_zNVXH-9Wi5YQ_MyYVt0tob0Zt_fGQ9itQWnDMRZJE5cRZGLBf4N8JfPrQ</recordid><startdate>19960301</startdate><enddate>19960301</enddate><creator>A Franco-Obregón</creator><creator>J López-Barneo</creator><general>The Physiological Society</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QP</scope><scope>7X8</scope><scope>5PM</scope></search><sort><creationdate>19960301</creationdate><title>Differential oxygen sensitivity of calcium channels in rabbit smooth muscle cells of conduit and resistance pulmonary arteries</title><author>A Franco-Obregón ; J López-Barneo</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c6021-dce61e2c348d73f2f4ae3ff309af7bfffd4fbbf7ed890bda463c67878d6b81523</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1996</creationdate><topic>Animals</topic><topic>Calcium Channels - drug effects</topic><topic>Calcium Channels - physiology</topic><topic>Hypoxia - physiopathology</topic><topic>Male</topic><topic>Membrane Potentials - drug effects</topic><topic>Membrane Potentials - physiology</topic><topic>Muscle, Smooth, Vascular - drug effects</topic><topic>Muscle, Smooth, Vascular - physiology</topic><topic>Oxygen - pharmacology</topic><topic>Pulmonary Artery - drug effects</topic><topic>Rabbits</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>A Franco-Obregón</creatorcontrib><creatorcontrib>J López-Barneo</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Calcium &amp; Calcified Tissue Abstracts</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>The Journal of physiology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>A Franco-Obregón</au><au>J López-Barneo</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Differential oxygen sensitivity of calcium channels in rabbit smooth muscle cells of conduit and resistance pulmonary arteries</atitle><jtitle>The Journal of physiology</jtitle><addtitle>J Physiol</addtitle><date>1996-03-01</date><risdate>1996</risdate><volume>491</volume><issue>Pt 2</issue><spage>511</spage><epage>518</epage><pages>511-518</pages><issn>0022-3751</issn><eissn>1469-7793</eissn><abstract>1. Calcium currents were recorded from smooth muscle cells dispersed from conduit and resistance rabbit pulmonary arteries. We tested the hypothesis that Ca2+ channel activity was regulated by environmental O2 tension. 2. Conduit (proximal) and resistance (distal) myocytes differ in their Ca2+ channel density and responses to low PO2. Ca2+ current density in distal myocytes (20.7 +/- 7.4 pA pF-1, n = 10) is almost twice the value in proximal myocytes (12.6 +/- 5.5 pA pF-1, n = 39). In proximal myocytes, the predominant response to reductions in PO2 is inhibition of the calcium current (n = 12) at membrane potentials below 0 mV, whereas potentiation of current amplitude is observed in distal myocytes (n = 24). 3. Hypoxia also produces opposite shifts in the conductance-voltage relationships along the voltage axis. The average displacements induced by low PO2 are +5.05 +/- 2.98 mV (n = 5) in proximal myocytes and -6.06 +/- 2.45 (n = 10) in distal myocytes. 4. These findings demonstrate longitudinal differences in Ca2+ channel density and O2 sensitivity in myocytes along the pulmonary arterial tree. These results may help to understand the differential reactivity to hypoxia of the pulmonary vasculature: vasodilatation in conduit arteries and vasoconstriction in resistance vessels.</abstract><cop>England</cop><pub>The Physiological Society</pub><pmid>8866874</pmid><doi>10.1113/jphysiol.1996.sp021235</doi><tpages>8</tpages><oa>free_for_read</oa></addata></record>
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subjects Animals
Calcium Channels - drug effects
Calcium Channels - physiology
Hypoxia - physiopathology
Male
Membrane Potentials - drug effects
Membrane Potentials - physiology
Muscle, Smooth, Vascular - drug effects
Muscle, Smooth, Vascular - physiology
Oxygen - pharmacology
Pulmonary Artery - drug effects
Rabbits
title Differential oxygen sensitivity of calcium channels in rabbit smooth muscle cells of conduit and resistance pulmonary arteries
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