Genetic Diversity of the Species of the Genus Deschampsia P.Beauv. (Poaceae) Based on the Analysis of the ITS Region: Polymorphism Proves Distant Hybridization

The species of the genus are difficult for identification, and the genus is difficult for taxonomic treatment. The regions of 35S rRNA genes were studied for the species of the genus of different geographical origin with a method of sequencing by Sanger (ITS1-5.8S rRNA gene-ITS2, 14 species) and with a method of a locus-specific next-generation sequencing (NGS) on the Illumina platform (ITS1-5.8S rRNA, 7 species). All species of formed one clade; the species, referred by some authors on the basis of morphological characters to the species s.l., entered one subclade. Subantarctic species formed a separate subclade and their ribotypes formed their own subnetwork. , earlier referred to , entered the other clade, though this species contains some ribotypes common with some species. and related mountain species have their own specific ribotype groups. On the network of the ribotypes, one can see that from Great Britain forms a network with some species, but from the USA forms its own network. Ribotype analysis of each sample revealed traces of introgression with in and with and probable introgression of Northern and subantarctic species.