The effect of mitogenic lectins and monoclonal antibodies on intracellular free calcium concentration in human T-lymphocytes

The effect of mitogenic lectins and the mitogenic antibody UCHT1 on the cytoplasmic free Ca2+ concentration ( [Ca2+]i) in human lymphocytes was investigated by using the fluorescent Ca2+-indicator quin2 . Phytohaemagglutinin, concanavalin A and UCHT1 increased [Ca2+]i in T-cells to a maximum level w...

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Veröffentlicht in:	Biochemical journal 1984-04, Vol.219 (2), p.661-666
Hauptverfasser:	O'Flynn, K, Linch, D C, Tatham, P E
Format:	Artikel
Sprache:	eng
Schlagworte:	Aminoquinolines Antibodies, Monoclonal - immunology Calcium - metabolism Concanavalin A - pharmacology Fluorescent Dyes Humans Lectins - pharmacology Lymphocyte Activation - drug effects Phytohemagglutinins - pharmacology Pokeweed Mitogens - pharmacology Spectrometry, Fluorescence T-Lymphocytes - metabolism
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Beschreibung
Zusammenfassung:	The effect of mitogenic lectins and the mitogenic antibody UCHT1 on the cytoplasmic free Ca2+ concentration ( [Ca2+]i) in human lymphocytes was investigated by using the fluorescent Ca2+-indicator quin2 . Phytohaemagglutinin, concanavalin A and UCHT1 increased [Ca2+]i in T-cells to a maximum level within 2 min. No T-cell response was seen with poke weed mitogen. None of the mitogens affected non-T-cell [Ca2+]i.
ISSN:	0264-6021 1470-8728
DOI:	10.1042/bj2190661