Effect of local anaesthetics on mitochondrial membrane potential in living cells

Using the laser dye rhodamine 123, we demonstrated that local anaesthetics can reach mitochondria in cell culture and reversibly decrease, or even collapse, their transmembrane potential. This effect is highly dependent on the lipid-solubility of the local anaesthetic and can be facilitated by the p...

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Veröffentlicht in:	Biochemical journal 1990-10, Vol.271 (1), p.269-272
Hauptverfasser:	Grouselle, M, Tueux, O, Dabadie, P, Georgescaud, D, Mazat, J P
Format:	Artikel
Sprache:	eng
Schlagworte:	Anesthetics, Local - chemistry Anesthetics, Local - pharmacology Animals Bupivacaine - chemistry Bupivacaine - pharmacology Cell Line Etidocaine - chemistry Etidocaine - pharmacology Fibroblasts - ultrastructure Fluorescent Dyes Intracellular Membranes - drug effects Intracellular Membranes - physiology Lidocaine - chemistry Lidocaine - pharmacology Membrane Lipids Membrane Potentials - drug effects Mice Microscopy, Fluorescence Mitochondria - drug effects Mitochondria - physiology Rhodamine 123 Rhodamines Solubility
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Beschreibung
Zusammenfassung:	Using the laser dye rhodamine 123, we demonstrated that local anaesthetics can reach mitochondria in cell culture and reversibly decrease, or even collapse, their transmembrane potential. This effect is highly dependent on the lipid-solubility of the local anaesthetic and can be facilitated by the presence of a lipophilic anion.
ISSN:	0264-6021 1470-8728
DOI:	10.1042/bj2710269