A stopped-flow calorimeter for biochemical applications

A stopped-flow calorimeter for biochemical applications

A rapid-response stopped-flow calorimeter for small samples of reagents is described. The construction, performance characteristics and operational limitations are described, along with an example of its ability to resolve the kinetics of an enzyme-catalysed hydrolysis. It is thought likely that the...

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Veröffentlicht in:Biochemical journal 1987-12, Vol.248 (3), p.677-682
Hauptverfasser: Howarth, J V, Millar, N C, Gutfreund, H
Format: Artikel
Sprache:eng
Schlagworte:
calorimetry
Calorimetry - instrumentation
Calorimetry - methods
Chymotrypsin - metabolism
Hydrolysis
Kinetics
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Zusammenfassung:A rapid-response stopped-flow calorimeter for small samples of reagents is described. The construction, performance characteristics and operational limitations are described, along with an example of its ability to resolve the kinetics of an enzyme-catalysed hydrolysis. It is thought likely that the method would find useful application in a variety of chemical and biochemical investigations.
ISSN:0264-6021
1470-8728
DOI:10.1042/bj2480677