Yoonia algicola sp. nov., Yoonia rhodophyticola sp. nov. and Yoonia phaeophyticola sp. nov., isolated from marine algae

Three Gram-stain-negative, strictly aerobic, non-motile, oxidase- and catalase-positive, short-rod-shaped bacteria, designated as strains G8-12 , SS1-5 and BS5-3 , were isolated from marine algae in South Korea. Strain G8-12 exhibited optimal growth at 20-25 °C, pH 8.0 and 2.0-2.5% (w/v) NaCl, while strains SS1-5 and BS5-3 grew optimally at 25 °C, pH 7.0 and 1.5% NaCl. All strains contained ubiquinone-10 as the sole respiratory quinone, with phosphatidylglycerol and phosphatidylcholine as major polar lipids, and C 7 and C as major fatty acids (>5 %); C 7 11-methyl and C 2-OH were additionally identified as major fatty acids in strain SS1-5 . The genomic DNA G+C contents were 57.0, 58.3 and 56.4% for strains G8-12 , SS1-5 and BS5-3 , respectively. Strains G8-12 , SS1-5 and BS5-3 exhibited less than 74.8% average nucleotide identity (ANI) and 19.7% digital DNA-DNA hybridization (dDDH) values with each other, indicating that they represent different species. Phylogenetic analyses based on both 16S rRNA gene and genome sequences revealed that strains G8-12 , SS1-5 and BS5-3 form distinct phylogenetic lineages within the genus . Relative to other closely related species, these strains exhibited ANI and dDDH values below 83.5 and 26.9%, respectively, suggesting that they constitute novel species within the genus . Based on their phenotypic, chemotaxonomic and phylogenetic characteristics, strains G8-12 , SS1-5 and BS5-3 represent three novel species of the genus , for which the names sp. nov. (G8-12 =KACC 22753 =JCM 35790 ), sp. nov. (SS1-5 =KACC 22649 =JCM 35753 ) and sp. nov. (BS5-3 =KACC 22648 =JCM 35751 ) are proposed, respectively.