Stimulated Brillouin scattering flow cytometry

We present the use of stimulated Brillouin scattering spectroscopy to achieve rapid measurements of cell biomechanics in a flow cytometer setup. Specifically, our stimulated Brillouin scattering flow cytometry can acquire at a rate of 200 Hz, with a spectral acquisition time of 5 ms, which marks a 1...

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Veröffentlicht in:	Biomedical optics express 2024-10, Vol.15 (10), p.6024-6035
Hauptverfasser:	Rosvold, Jake R, Zanini, Giulia, Handler, Chenchen, Frank, Eric, Li, Jiarui, Vitolo, Michele I, Martin, Stuart S, Scarcelli, Giuliano
Format:	Artikel
Sprache:	eng
Online-Zugang:	Volltext
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Zusammenfassung:	We present the use of stimulated Brillouin scattering spectroscopy to achieve rapid measurements of cell biomechanics in a flow cytometer setup. Specifically, our stimulated Brillouin scattering flow cytometry can acquire at a rate of 200 Hz, with a spectral acquisition time of 5 ms, which marks a 10x improvement compared to previous demonstrations of spontaneous Brillouin scattering flow cytometry. We experimentally validate our stimulated Brillouin scattering flow cytometer by measuring cell populations of normal breast epithelial cells and metastatic breast epithelial cancer cells.
ISSN:	2156-7085 2156-7085
DOI:	10.1364/BOE.537602