Stimulation of Local Cytosolic Calcium Release by Photothermal Heating for Studying Intra‐ and Intercellular Calcium Waves
A methodology is described that allows for localized Ca2+ release by photoexcitation. For this, cells are loaded with polymer capsules with integrated plasmonic nanoparticles, which reside in endo‐lysosomes. The micrometer‐sized capsules can be individually excited by near‐infrared light from a ligh...
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Veröffentlicht in: | Advanced materials (Weinheim) 2021-06, Vol.33 (24), p.e2008261-n/a |
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Sprache: | eng |
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Zusammenfassung: | A methodology is described that allows for localized Ca2+ release by photoexcitation. For this, cells are loaded with polymer capsules with integrated plasmonic nanoparticles, which reside in endo‐lysosomes. The micrometer‐sized capsules can be individually excited by near‐infrared light from a light pointer, causing photothermal heating, upon which there is a rise in the free cytosolic Ca2+ concentration ([Ca2+]i). The [Ca2+]i can be analyzed with a Ca2+ indicator fluorophore. In this way, it is possible to excite local lysosomal Ca2+ release in a desired target cell.
A methodology is reported to trigger intra‐ and intercellular calcium waves by photoexcitation. Polymeric capsules with integrated plasmonic nanoparticles inside lysosomes are individually excited by near‐infrared light to transiently release small amounts of Ca2+, which can evoke abundant Ca2+ release from the endoplasmic reticulum, leading to further propagating intra‐ and intercellular calcium waves. |
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ISSN: | 0935-9648 1521-4095 1521-4095 |
DOI: | 10.1002/adma.202008261 |